关键词: 欧李, 番茄, 八氢番茄红素合成酶基因, 遗传转化, 类胡萝卜素

Abstract: The ChPSY cDNA from the Cerasus humilis fruit was analyzed，digested and inserted into plant expression vector PMV，resulting in the plant recombinant vector pBI-ChPSY，which was introduced into tomato via Agrobacterium-mediated transformation. Twelve putatively independent transgenic plants were generated. PCR and Southern blot analysis showed 8 out of these putative transgenic lines were positive，which revealed that ChPSY gene was integrated into the transgenic lines genome. RT-PCR analysis indicated that ChPSY gene was expressed in the fruits of these transgenic lines. HPLC analysis showed that the total amount of carotenoids in the fruit of transgenic tomato was 1.62 to 3.04-fold higher than the non-transformed controls，at the same time，phytoene，lycopene，β-carotene and α-carotene levels were also increased by 4.87，2.10，2.59 and 3.25 folds，respectively. In addition，the expression of endogenous genes involved in carotenoid synthesis was affected in the transgenic lines. These results suggested that overexpression of ChPSY significantly enhanced the carotenoids biosynthesis and accumulation in transgenic tomato fruits.

Key words: Cerasus humilis, tomato, phytoene synthease gene, genetic transformation, carotenoids