关键词: 大白菜, 结球甘蓝, 小孢子培养, 易位系, InDel标记

Abstract: In order to create Chinese cabbage–cabbage translocation lines，isolated microspores of Chinese cabbage–cabbage monosomic addition lines 3#（AA + C3）were cultured. Through identifying the obtained microspore plants by specific InDel markers located on Linkage group C02 of cabbage，one plant which has cabbage specific bands was identified from seventeen microspore plants. By designing multiple InDel markers around the targeted region，the exogenous cabbage fragment size is clarified as 1.03 Mb in this plant. Using one hundred InDel markers uniformly distributed on ten A genome linkage groups of Chinese cabbage，the chromosome fragment of cabbage was preliminary determined on the 5# chromosome of Chinese cabbage. Meiosis behavior of this translocation line showed that the chromosome number of this plant is twenty，which is identified as one translocation line introduced one segment of chromosome 3# in cabbage.

Key words: Chinese cabbage, cabbage, microspore culture, translocation line, InDel markers