https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2014, Vol. 41 ›› Issue (7): 1344-1354.

• 蔬菜 • 上一篇    下一篇

与甘蓝中心柱长相关联的EST-SSR标记分析

孙朋朋1,2,刘基生2,张扬勇2,方智远2,刘玉梅2,杨丽梅2,龚义勤1,*,
柳李旺1,李占省2,庄 木2,*   

  1. 1 南京农业大学园艺学院,南京 210095;2 中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2014-07-25 发布日期:2014-07-25
  • 基金资助:
    国家重点基础研究发展计划(‘973’)项目(2012CB1139);国家现代农业产业技术体系建设专项资金项目(CARS-25);国家‘863’计划课题(2012AA100101,2012AA100202);农业部园艺作物生物学与种质创制重点实验室项目

Analysis on EST-SSR Markers Associated with Core Length in Cabbage

  • Online:2014-07-25 Published:2014-07-25

摘要: 利用40 个EST-SSR 标记,对100 份甘蓝自交系组成的自然群体进行遗传结构分析,采用
TASSEL3.0 软件的GLM(general linear model)和MLM(mixed linear model)模型,对甘蓝中心柱长和
中心柱长/球高比值性状进行关联分析。结果显示供试材料可分为3 个亚群;EST-SSR 位点间有较高的多
态性和一定程度的连锁不平衡;以GLM 模型分析,共检测出5 个标记的7 个位点与中心柱长性状相关联,
2 个标记的3 个位点与中心柱长/球高性状相关联,其中2 个位点同时与两个性状相关联;以MLM 模型分
析,共检测出3 个标记的4 个位点与中心柱长性状相关联,1 个标记的2 个位点与中心柱长/球高性状相
关联,其中2 个位点同时与两个性状相关联。GLM 和MLM 两种模型同时检测到了与中心柱长/球高相关
联的1 个标记的2 个位点以及与中心柱长相关联的3 个标记的4 个位点。

关键词: 甘蓝, EST-SSR, 遗传多样性, 群体结构, 关联分析

Abstract: Forty EST-SSR markers were applied for the genetic structure analysis of a natural population comprised 100 cabbage inbred lines,and association analysis between markers and two agronomic traits on core length were subsequently performed using GLM(general linear model)and MLM(mixed linear model)programs of TASSEL3.0 software in this study. Population genetic structure analysis showed that 100 cabbage accessions were composed of 3 subpopulations,and high polymorphism and various degree of linkage disequilibrium were detected among EST-SSR loci. There were 7 alleles of 5 markers associated with core length,and 3 alleles of 2 markers associated with core length/vertical diameter of head,and 2 alleles associated simultaneously with two observed traits under GLM program. There were 4 alleles of 3 markers associated with core length,and 2 alleles of 1 marker associated with core length/vertical diameter of head,and 2 alleles associated simultaneously with two observed traits under MLM program. Furthermore,2 alleles of 1 marker associated with core length/vertical diameter ofhead and 4 alleles of 3 markers associated with core length were determined under GLM and MLM programs.

Key words: cabbage, EST-SSR, genetic diversity, population structure, association analysis

中图分类号: