关键词: 杧果, MiRab11, 克隆, 表达分析

Abstract: A MiRab11 gene，which belonged to small GTP-binding proteins Rab gene family，was
cloned by RT-PCR and RACE from Mangifera indica mixed tissues（leaves，stems，flowers，and fruits）.
The full-length cDNA sequence was 902 bp and contained an open reading frame of 654 bp，which
encoded a 218 amino acid protein. The deduced amino acid sequence exhibited high homology with Vitis
vinifera and Citrus sinensis（97% similarity）. Real-time quantitative PCR detection showed that the
ubiquitously expressed MiRab11 in young stems and fruit of 70 d after flowering was much higher than in
other tissues；The expression level of the gene was down-regulated during early fruit development from 20
d to 50 d after flowering and up-regulated during fruit ripening from 50 d to 70 d after flowering；It could
be up-regulated under different stress treatments（low temperature，salt，and drought）and stimulated by
different signal molecules stimulation（abscisic acid，salicylic acid，and hydrogen peroxide）. In a word，
a MiRab11 was identified from Mangifera indica，which might be associated with mango fruit ripening and play an important role in mango stress reaction.

Key words: Mangifera indica, MiRab11, cloning, expression analysis