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园艺学报 ›› 2014, Vol. 41 ›› Issue (6): 1198-1206.

• 研究报告 • 上一篇    下一篇

野生毛花猕猴桃果实表型性状及SSR遗传多样性分析

汤佳乐,黄春辉,吴 寒,郎彬彬,曲雪艳,徐小彪*   

  1. (江西农业大学农学院,南昌 330045)
  • 收稿日期:2013-12-11 出版日期:2014-06-25 发布日期:2014-06-25

Genetic Diversity of Wild Actinidia eriantha Germplasm Based on Fruit Traits and SSR Markers

TANG Jia-le,HUANG Chun-hui,WU Han,LANG Bin-bin,QU Xue-yan,and XU Xiao-biao*   

  1. (College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China)
  • Received:2013-12-11 Online:2014-06-25 Published:2014-06-25

摘要: 以江西省武功山境内的70份野生毛花猕猴桃种质资源为试材,对其果实表型性状、SSR遗传多样性及其亲缘关系进行分析和评价。根据UPOV(国际植物新品种保护联盟)公布的猕猴桃属品种测定标准对供试材料的果实表型性状进行观测。参照猕猴桃遗传连锁图谱,选用分布于中华猕猴桃基因组中的70对SSR引物对供试材料进行多态性分析。结果表明,在70对引物中,21对引物成功扩增出多态性片段。随机采集的70份野生毛花猕猴桃种质资源中,其果实表型性状和DNA分子水平均存在丰富的遗传多样性。基于UPGMA聚类分析将供试的野生毛花猕猴桃资源分为果实圆形和椭圆形混合组、果实椭圆形组以及果实圆柱形组。21对多态性高的SSR引物共检测出127个等位变异位点,变异范围在2 ~ 12之间,平均每对SSR引物可检测到6.04个等位位点。遗传相似系数(GS)变异范围为0.5306 ~ 0.9252。GS值在0.65水平上UPGMA聚类分析可将供试的野生毛花猕猴桃种质资源划分成Ⅰ、Ⅱ和 Ⅲ 共3个组,这与果实表型性状分析的结果基本一致。这些遗传变异丰富的种质资源可为猕猴桃育种提供有价值的材料。

关键词: 毛花猕猴桃, 野生, SSR标记, 果实表型性状, 遗传多样性

Abstract: Genetic diversity and genetic relationship of 70 Actinidia eriantha Benth. germplasms sampled from Wugong Mountain in Jiangxi Province were estimated based on the fruit morphological traits and SSR markers. The fruit phenotypic traits were collected based on the UPOV(International Union for the Protection of New Varieties of Plants)for kiwifruit. Seventy SRR primer pairs even distributed on genetic linkage map of Actinidia chinensis were used to initially assess the polymorphism. Of the 70 primer pairs tested,21 pairs successfully amplified polymorphic fragments. The collected 70 accessions have expressed rich genetic diversity in both fruit traits and SSR markers. Seventy accessions were clustered into three groups including the circular and elliptic group,elliptic group and the oblong group based on UPGMA analysis. One hundred and twenty-seven bands were amplified using 21 primer pairs,and the number of allele per locus ranged from 2 to 12,with an average of 6.04. The genetic similarity ranged from 0.5306 to 0.9252. Upon on the UPGMA cluster analysis,70 accessions were separated into three major groups when GS values were set at the level of 0.65,which was basically consistent with the results of the fruit traits. The high level of genetic variation of A. eriantha suggested that these accessions may be useful to provide breeding materials for kiwifruit.

Key words: Actinidia eriantha, wild, SSR marker, fruit morphological traits, genetic diversity

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