关键词: 羽衣甘蓝, 小孢子, 胚状体, 再生植株

Abstract: Plant regeneration was firstly reported bymeans of isolated microspore culture in edible kale. A hybrid cultivar named‘Succulent Green Leaf’which was introduced from Japan was used for the experiment. Several combinatory were screened in microspore derived embryos, trans-embryos time, hormone constitution on shooting and rooting in order to obtain the bestway on the regenerative system. Embryoswere attained in the mediums of NLN-13 + 0.1 mg·L ^-1 6-BA + 0.1 mg·L^-1 NAA ( embryo yield per bud was 1.38) and NLN-13 + 0.2 mg·L^-1 62BA + 0.2 mg·L^-1 NAA ( the yield was 0.98). The suitable time for transfer of the embryos was 25 days after inoculation; The best shooting medium was MS+2.0 mg·L^-1 6-BA+ 0.1 mg·L^-1 NAA + 3% sugar + 0.7% agarose. With the shoot induction rate was 56.38% , the suitable medium for rooting was 1/2MS + 0.1 mg·L^-1NAA + 3% sugar + 0.7% agarose and the root inducing rate come up to 100%.

Key words: kale, microspore, embryo, plant regeneration