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园艺学报 ›› 2014, Vol. 41 ›› Issue (5): 1009-1019.

• 综述 • 上一篇    下一篇

葡萄病毒分子检测技术研究进展

范旭东,董雅凤*,张尊平,任 芳,胡国君,朱红娟   

  1. 中国农业科学院果树研究所国家落叶果树脱毒中心,辽宁兴城 125100
  • 出版日期:2014-05-25 发布日期:2014-05-25
  • 基金资助:

    国家现代农业产业技术体系建设专项资金项目(CARS-30-bc-3)

Progress on Molecular Detection of Grapevine Viruses

FAN Xu-dong,DONG Ya-feng*,ZHANG Zun-ping,REN Fang,HU Guo-jun,and ZHU Hong-juan   

  1. National Center for Eliminating Viruses from Deciduous Fruit Tree,Research Institute of Pomology,Chinese Academy of
    Agricultural Sciences,Xingcheng,Liaoning 125100,China
  • Online:2014-05-25 Published:2014-05-25

摘要: 简述了葡萄病毒RT-PCR、分子杂交、基因芯片、RT-LAMP 以及高通量测序等分子检测技
术的原理和特点,综述了近年来葡萄病毒分子检测所取得的进展,展望了今后研究的主要方向。

关键词: 葡萄, 病毒, 分子检测, 研究进展

Abstract: In this paper,the molecular detection technologies used for grapevine viruses in recent
years are outlined,which include RT-PCR,molecular hybridization,gene microarray,RT-LAMP and
high-through sequence,and the achievements of those technologies in the detection of grapevine virus are
summarized. Furthermore,the further research directions of molecular detection of grapevine viruses are
also discussed.

Key words: grapevine, virus, molecular detection, research advance

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