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园艺学报 ›› 2014, Vol. 41 ›› Issue (5): 985-993.

• 研究报告 • 上一篇    下一篇

成团泛菌 Ljb-2 对番茄黄化曲叶病毒病的田间防效初步研究

柯红娇 1,王勇 1,卫甜 1,谷春 2,刘红霞1,郭坚华1,*   

  1. 1 南京农业大学植物保护学院植物病理学系,江苏省生物源农药工程中心,农业部作物病虫害监测与防控重点开放
    实验室,南京 210095;2 江苏省安丰生物源农药工程中心,江苏太仓 215400
  • 出版日期:2014-05-25 发布日期:2014-05-25
  • 基金资助:

    农业部公益性行业专项(201003065)

Suppression of Tomato Yellow Leaf Curl Virus Disease by the Pantoea agglomerans Strain Ljb-2 in the Field

  1. 1Department of Plant Pathology,College of Plant Protection,Nanjing Agricultural University,Key Laboratory of
    Integrated Management of Crop Diseases and Pests(Nanjing Agricultural University),Ministry of Education,Nanjing
    210095,China;2Anfeng Biogenic Pesticide Engineering Center of Jiangsu Province,Taicang,Jiangsu 215400,China
  • Online:2014-05-25 Published:2014-05-25

摘要: 成团泛菌(Pantoe agglomerans)Ljb-2 是从番茄生境中分离得到的对番茄黄化曲叶病毒病
(tomato yellow leaf curl virus disease,TYLCD)具有较好防效的拮抗细菌,其对温室中番茄感病品种‘格
瑞斯’TYLCD 的防控效果为49.77%。2012 年4—8 月在连云港进行的田间试验结果表明,该菌株在田间
条件下对感病品种‘格瑞斯’TYLCD 的防效达到57.12%。2012 年7—12 月,将该菌株进行田间防治试
验,结果表明,番茄移栽60 d 时,Ljb-2 对番茄中抗品种‘迪芬妮’TYLCD 的防效达到45.26%。同时,
用Ljb-2 处理番茄植株后,能显著增加叶片叶绿素a、叶绿素b 及总叶绿素含量,提高果实中维生素C、
可溶性糖、可滴定酸含量及糖酸比,对果实品质具有明显的改善作用。此外,对番茄的增产效果达到
20.05%。

关键词: 番茄, 番茄黄化曲叶病毒病, 生物防治, 烟粉虱, 成团泛菌

Abstract: Aiming at developing biological control agents(BCAs)that can effectively suppress tomato
yellow leaf curl virus disease(TYLCD),this study investigated the biocontrol efficacy of the Pantoea
agglomerans strain Ljb-2 against TYLCD as well as its plant-growth-promoting effect under both
greenhouse and field conditions. Ljb-2 was originally isolated from the tomato environment. Applied to the
susceptible tomato variety Geruisi with root drenching in combination with foliar spray,it achieved the
significant biocontrol efficacy of 49.77% and 57.12% against TYLCD in the greenhouse and the field,
respectively,in spring 2012. At 60 d post transplantation in the field experiment in fall 2012,with the
same application method,Ljb-2 attained the biocontrol efficacy of 45.26% against TYLCD on Defenni (a tomato variety with moderate resistance to TYLCD). Ljb-2 significant increased the contents of
leaf chlorophylls a,b,and a + b in the field,elevated tomato yield by 20.05%,leading to rises in the
contents of vitamin C,soluble sugar,titratable acid,and sugar-acid ratio in tomato fruit in the field in
fall 2012.

Key words: tomato, Tomato yellow leaf curl virus, biocontrol, Bemisia tabaci, Pantoe agglomerans

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