关键词: 茄子, NAC, 克隆, 基因表达, 低温, 高盐, 赤霉素

Abstract: Eggplant seedlings were used as experimental materials and the full-length of SmNAC1
was cloned using Rapid-amplification of cDNA ends（RACE）. The results showed that the full-length
sequence of SmNAC1 is 1 279 bp and the open reading frame is 909 bp which encoding a polypeptide of
302 amino acids with a protein molecular weight of about 35.04 kD. SmNAC1 contains the N-terminal
conserved domain of NAC family and homology was as high as 90% with tomato and potato. The
expression patterns were analyzed by Real-time quantitative PCR（RT-qPCR）under GA，4 ℃ and NaCl
treatment. The results showed that SmNAC1 was induced by GA and stress such as low temperature and
high salt treatment. However，there were some differences of SmNAC1 gene expression in roots，stems and
leaves. The overall level of expression in the roots was higher than stems and leaves under different
treatments，which indicated that SmNAC1 was predominantly expressed in root. The relative expression
level of SmNAC1 reached a high level with short time（0.5–1 h），while the reaction was relatively slow in
stems and leaves. The relative expression level had the smallest variations in leaves under different treat
times. The results suggested SmNAC1 may participate response of GA-induced and adjustment of low
temperature and high salt stress tolerance in eggplant.

Key words: eggplant, NAC, cloning, gene express, low temperature, high salt, GA