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园艺学报 ›› 2014, Vol. 41 ›› Issue (5): 898-906.

• 蔬菜 • 上一篇    下一篇

甜瓜果实颜色3 个质量性状基因的定位

杨光华 1,范 荣1,杨小锋1,侯军亮2,袁士臣3,曹 明1,王学林1,
李劲松1,*   

  1. 1 三亚市南繁科学技术研究院/南繁育种海南省工程实验室,海南三亚 572000;2 深圳华大基因研究院,广东深圳 518083;3 新疆宝丰种业有限公司,新疆昌吉 831100
  • 出版日期:2014-05-25 发布日期:2014-05-25
  • 基金资助:

    海南省重大科技项目(ZDZX2013010);海南省应用技术研究与开发专项(ZDXM2014044)

Construction of a Highly Dense Genetic Map Using SNP and Mapping of Three Qualitative Traits in Cucumis melo

1Sanya Science & Technology Academy For Crop Winter Multiplication/Hainan Engineering Laboratory for Crop Winter
Multiplication and Breeding,Sanya,Hainan 572000,China; 2 Beijing Genomics Institute-Shenzhen,Shenzhen,Guangdong
518083,China;3Xinjiang Baofeng Seeds Co.,Ltd,Changji,Xinjiang 831100,China   

  • Online:2014-05-25 Published:2014-05-25

摘要: 以甜瓜(Cucumis melo L.)黄绿皮、白肉、无覆纹的自交系‘K1-7’为母本,黄皮、橘红肉、
无覆纹的自交系‘K3-92-1’为父本杂交,组建F2 遗传群体,采用MSG(multiplexed shotgun genotyping)
法对F2 群体进行基因分型,并在甜瓜全基因组范围内扫描SNP 遗传标记。利用检测到的44 722 个SNP
标记位点进行高密度Bin 遗传图谱的构建,并对甜瓜果皮底色、覆纹颜色和果肉颜色等3 个质量性状进行
遗传分析和基因定位。结果表明,果皮底色和果肉颜色由单基因控制,黄绿果皮和橘红色果肉为显性性
状,果皮底色基因定位在4 号染色体409 828 ~ 835 625 bp 区间内,长度约为425 kb,果肉颜色基因定位
在9 号染色体20 433 942 ~ 20 573 889 bp 区间内,长度约为139 kb,覆纹颜色由两个有上位效应的基因控
制,其中1 个基因位点与果皮颜色控制位点在同一区域,1 个基因定位在2 号染色体末端23 736 803 ~
23 787 235 bp 区间内,长度约为51 kb。

关键词: 甜瓜, 果皮底色, 果皮覆纹, 果肉颜色, SNP 标记, 基因定位

Abstract: A Bin map of melon was constructed using a F2 population developed from a crossing the
melon inbred lines between‘K1-7’(green-yellow primary skin color,white flesh color and absence
secondary skin color)and‘K3-92-1’(yellow primary skin color,red-orange flesh color and absence
secondary skin color). A genotyping method based on restriction named MSG was applied to genotype for
the F2 population,and then SNPs had been scaned in the genome-wide of melon. The Bin map included 44 722
SNPs,and then genetic analysis and gene maping had been done for primary skin color,secondary skin
color and flesh color. Genetic analysis showed that the primary skin color and flesh color is determined by a single gene separately,in addition,the character of green-yellow primary skin color and red-orange flesh
color is dominant inheritance. The genetic loci control of primary skin color was mapped on chromosome
4 of melon,narrowed the range of 409 828 bp to 835 625 bp(425 kb region),the genetic loci control of
flesh color was mapped on chromosome 9,narrowed the range of 20 433 942 bp to 20 573 889 bp(139 kb
region). The genetic control of secondary skin color was found to be complex,involving two loci with
epistatic interactions. One of these loci was the same region as the genetic loci control of primary skin
color trait,the other gene loci was mapping on chromosome 2,narrowed the range of 23 736 803 bp to
23 787 235 bp(51 kb region).

Key words: melon, primary skin color, secondary skin color, flesh color, SNP marker, gene mapping

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