关键词: 结球甘蓝, 同源异型盒蛋白, 基因克隆, 表达分析

Abstract: Four significantly differentially expressed HB（homeobox）analogs genes，BoHAT2，
BoHB12，BoHB7，BoHB27 were identified through transcriptome comparison of stem tip and leaf at
rosette stage and heading stage of cabbage（Brassica oleracea L.‘519’）. BoHAT2，BoHB12，BoHB7，BoHB27 and else three HB analogs genes BoPHB，BoPHV，BoREV，which were no transcription difference
at rosette stage and heading stage of cabbage but had been proved involving in the process of leaf curl in
Arabidopsis thaliana were isolated respectively using homologous cloning method. Amino acid sequence
analysis showed that seven genes share a homeodomain of homeobox family. BlastP analysis indicated all
of proteins are highly homologous with HB protein family from Arabidopsis thaliana. The similarity of
amino acid sequence of BoHAT2，BoHB12，BoHB7，BoHB27，BoPHB，BoPHV，BoREV genes are 86%，
80%，79%，60%，94%，95%，96% compared with HAT2，ATHB12，ATHB-7，ATHB27，PHB，PHV，
REV from Arabidopsis thaliana，respectively. Further BlastP and phylogenetic tree analysis indicated that
BoHAT2 belongs to HD-Zip Ⅱ protein，BoHB12 and BoHB7 belong to HD-Zip protein，BoHB27 belongs
to ZF-HD protein，BoPHB，BoPHV and BoREV belong to HD-Zip Ⅲ protein. The real-time PCR analysis
indicated the BoHAT2，BoHB12，BoHB7，BoHB27，BoPHB，BoPHV and BoREV genes express in stem
tip and leaf from rosette stage to heading stage. The BoHB12 and BoHB7 genes expressed higher in the
leaf of heading stage than the leaf of rosette stage，there were 38.1 times more BoHB12 transcription at
heading stage than rosette stage and 6.2 times more BoHB7 transcription at heading stage than rosette stage
were detected. No obvious expression difference was observed for else five genes between heading stage
and rosette stage. These results demonstrate the BoHB12 and BoHB7 genes may be the major genes that
involved in the process of cabbage leaf curl.

Key words: Brassica oleberacea, homeobox, gene cloning, expression analysis