Abstract: Chromosomal localization of 45S rDNA and 5S rDNA were studied by means of
fluorescence in situ hybridization（FISH）on metaphase chromosomes of Rubus ellipticus，R. pinfaensis and
R. ellipticus var. obcordatus. Genomic in situ hybridization（GISH）technique was also used to analyze the
phylogenetic relationship among them. The results showed that two 45S rDNA and two 5S rDNA sites
were detected in the three taxa. All 45S rDNA sites were localized on terminal chromosomal regions. 5S
rDNA loci localized on peri-centromeric region of short arms in R. ellipticus and R. pinfaensis，but located
on terminal regions in R. ellipticus var. obcordatus. GISH results indicated that the genomes of R.
ellipticus and R. pinfaensis shared many common DNA sequences on 14 chromosomes. Eight weak signals
around centromere and telomere were shared between R. ellipticus and R. ellipticus var. obcordatus.
Meanwhile，R. pinfanesis showed close relationship with R. parvifolius from subsection Stimulantes，distant with R. coreanus from subsection Pungentes. Combined the above data and morphological
differences，we approved the view that R. ellipticus and R. pinfaensis should be combined and placed the
combined species R. ellipticus into subsection Stimulantes. R. ellipticus var. obcordatus should be treated
as R. obcordatus rather than as a variety of R. ellipticus.

Key words: Rubus ellipticus, Rubus pinfaensis, Rubus ellipticus var. obcordatus, fluorescence in situ hybridization（FISH）, genomic in situ hybridization（GISH）, taxonomic treatments