关键词: 柑橘, 启动子, GUS 基因, CsTBL1 基因

Abstract: A 2 361 bp long promoter of the CsTBL1 gene was isolated from sweet orange[Citrus
sinensis（L.）‘Olinda’]. Sequence analysis showed that the promoter harbors multiple stress-responsive
cis-elements. To gather clues of the specific functions of the promoter a promoter-reporter vector was
constructed，and introduced it into Arabidopsis by floral dip method. Analysis of T3 transgenic progenies
with histochemical staining showed that high GUS activity in whole plants of 1- and 3-day-old seedlings.
Strong GUS activity was still detected in cotyledons and roots of 7- and 20-day-old seedlings，but
disappeared in hypocotyls. In 50-day-old seedlings，however，GUS activity was only detected in root tips
and trichomes. GUS expression was induced by treatments with ethylene precursor ACC，ABA，MeJA，and SA，suggesting that CsTBL1 could be involved in plant development and stress responses.

Key words: citrus, promoter, GUS gene, CsTBL1 gene