关键词: 冬枣, ACC氧化酶, 果实, 成熟, 基因, 克隆, 表达

Abstract:

Two ACC oxidase（ACO）cDNA fragments were isolated from half-red‘Dongzao' jujube fruit by 3′ RACE technique using degenerate primers corresponding to the conserved nucleotide and amino acid region sequences of the ACO family, then the 5′ end encoding sequences of both cDNAs were obtained by RT-PCR experiments. The two cDNAs, named as ZjACO1 and ZjACO2, contain an ORF（open reading frame）with 3′UTR（untranslated region）respectively. Their accession numbers in GenBank are EU216549 and EU216550. The two cDNAs are 1 115 bp and 1 105 bp in length, while the predicted proteins of them are 319 and 321 aa long, respectively. The similarity between ZjACO1 and ZjACO2 is 79.4% at nucleotide level, and 84.0% at amino acid level. The result of Northern blot analysis indicated that expressions of two cDNAs in jujube leaves were both undetectable, while their expressions at the development and ripening stages of jujube fruit were obviously different.

Key words: Ziziphus jujuba, jujube, ACC oxidase, fruit, ripening, gene, cloning, expression