关键词: 黄瓜, 果皮蜡质, CsCER7, 序列分析, 表达分析

Abstract: CsCER7，a homology exonucleases gene of Arabidopsis thaliana CER7，was recently cloned from cucumber（Cucumis sativus L.）. Phylogenetic reconstruction indicated that CsCER7 has closer relation with SlCER7. CsCER7 expression was characterized by RT-PCR，GUS staining，in suit hybrid（ISH）. The results showed that CsCER7 is expressed in tested organs，especially in the epidemic cells of fruits. A GFP fusion protein analysis showed that CsCER7 protein is localized in the nucleus and partial cytoplasm where AtCER7 protein is also localized previously reported. Promoter prediction showed that it contains several cis-acting elements such as MYCs and ABREs which response to ABA. RT-PCR analyses that expression of CsCER7 in cucumber fruit peel is increased under treatment with ABA. And wax load of fruit is also improved upon ABA treatment. Collectively，these results indicated that CsCER7 acts response to ABA which induces its higher expression，and further changes the accumulation of fruit cuticular wax.

Key words: cucumber, pericarp wax, CsCER7, sequence analysis, expression analysis