瑞典常春藤漆斑病病原鉴定

园艺学报 ›› 2009, Vol. 36 ›› Issue (4): 553-558.

瑞典常春藤漆斑病病原鉴定

贲海燕^1,2;李宝聚^1*;刘学敏²;石延霞¹

(¹中国农业科学院蔬菜花卉研究所, 北京100081; ²东北农业大学农学院, 哈尔滨150030)

收稿日期:2008-11-28 修回日期:2009-04-07 出版日期:2009-04-25 发布日期:2009-04-25
通讯作者: 李宝聚

Identification of the Pathogen Causing Black SpotD isease of Little Spurflower

BEN Hai-yan^1,2,LI Bao-ju^1*,LIU Xue-min²,and SHI Yan-xia¹

(¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, B eijing 100081, China; ²Agronomy College,Northeast Agricultural University, Harbin 150030, China)

Received:2008-11-28 Revised:2009-04-07 Online:2009-04-25 Published:2009-04-25
Contact: LI Bao-ju

摘要/Abstract

摘要： 通过病原菌的形态特征、培养性状和致病性测定的方法对引起瑞典常春藤漆斑病的病原菌进
行了分离鉴定, 采用PCR技术扩增病菌rDNA2ITS基因, 获得一长度为523 bp的DNA片段, 序列测定结果表明该片段序列与GenBank已有的露湿漆斑菌序列的同源性达到了99%。综合两种方法鉴定结果, 露湿漆斑菌是引起瑞典常春藤漆斑病的致病菌, 露湿漆斑菌为该寄主上的真菌新病害。

关键词: 瑞典常春藤, 瑞典常春藤漆斑病, 露湿漆斑菌, ITS-rDNA测序

Abstract: A pathogenic fungus causing leaf spot of Plectranthus parviflorus was isolated and characterized based on the morphological features, cultural characteristics and pathogenicity. By using polymerase chain reaction ( PCR) technique to amp lify rDNA2ITS of the isolate, we obtained a 523 bp DNA fragment, the result showed that rDNA-ITS sequences of the isolate and Myrothecium roridum shared 99% homology, which was compared in National Center for Biotechnology Information (NCBI) GenBank. Synthesized two identification results indicated that Myrothecium roridum was the pathogen causing black spot disease of Plectranthus parvif lorus. This is the first report of Myrothecium leaf spot caused by Myrothecium roridum on Plectranthus
parviflorus.

Key words: Plectranthus parviflorus, little spurflower black spot disease, Myrothecium roridum Tode exFr., ITS-rDNA sequencing

中图分类号:

S 682.36

贲海燕;李宝聚;刘学敏;石延霞. 瑞典常春藤漆斑病病原鉴定[J]. 园艺学报, 2009, 36(4): 553-558.

BEN Hai-yan;LI Bao-ju;LIU Xue-min;and SHI Yan-xia. Identification of the Pathogen Causing Black SpotD isease of Little Spurflower[J]. ACTA HORTICULTURAE SINICA, 2009, 36(4): 553-558.