异源表达唐菖蒲GhOPR3 提高了拟南芥的抗逆性

园艺学报 ›› 2014, Vol. 41 ›› Issue (3): 498-508.

异源表达唐菖蒲GhOPR3 提高了拟南芥的抗逆性

连青龙 1,2，辛海波2，李晓昕2，钟雄辉2，尹义蕾1，义鸣放2,*

1 农业部规划设计研究院设施农业研究所，农业部农业设施结构工程重点实验室，北京 100125；2 中国农业大学观
赏园艺与园林系，北京 100193

出版日期:2014-03-25 发布日期:2014-03-25
基金资助:
公益性行业（农业）科研专项（200903002）；国家自然科学基金项目（31171991）

Heterologous Expression of Gladiolus GhOPR3 Enhances the Abiotic Stress Resistance of Arabidopsis

LIAN Qing-long1,2，XIN Hai-bo2，LI Xiao-xin2，ZHONG Xiong-hui2，YIN Yi-lei1，and YI Ming-fang2,*

1Institute of Facility Agriculture，Chinese Academy of Agricultural Engineering，Key Laboratory of Farm Building in
Structure and Construction，Ministry of Agriculture，Beijing 100125，China；2Department of Ornamental Horticulture and
Landscape Architecture，China Agricultural University，Beijing 100193，China

Online:2014-03-25 Published:2014-03-25

摘要/Abstract

摘要： 通过RT-PCR 与RACE 技术从唐菖蒲（Gladiolus hybridus）品种‘Rose Supreme’球茎中克
隆茉莉酸生物合成途径中的关键酶——12–氧–植物二烯酸还原酶（12-oxo-phytodienoic acid reductase，
OPR）基因的1 489 bp 全长cDNA 序列，quantitative RT-PCR 结果表明，GhOPR3 在唐菖蒲叶、花、根、
匍匐茎、新球茎和籽球中都表达，其中在籽球和匍匐茎中相对表达量较高；0.1 ~ 0.5 mmol · L-1 的茉莉酸
甲酯（Methyl jasmonate，MJ）处理后，提高了GhOPR3 在球茎中的表达量和内源MJ 含量；采用农杆菌
介导侵染拟南芥花粉，进行GhOPR3 基因的过表达分析，过表达拟南芥株系较野生型提高了耐盐性和抗
旱性；提高了机械损伤后相关基因的表达水平和内源MJ 含量。

关键词: 唐菖蒲；茉莉酸；12&ndash, 氧&ndash, 植物二烯酸还原酶；异源表达；抗逆性

Abstract: OPR is a key enzyme in jasmonic acid biosynthesis pathway，a full-length cDNA named
GhOPR3 was cloned in Gladiolus hybridus‘Rose Supreme’corms by RT-PCR and RACE. The results of
quantitative RT-PCR showed that GhOPR3 gene was expressed in leaf，flower，root，stolon，corm and
cormel，and the relatively high expression level of GhOPR3 was observed in cormel and stolon.
Meanwhile，the expression level and the endogenous MJ content in corms steadily increased under MJ
treatment with a raising concentration gradients from 0.1 mmol · L-1 to 0.5 mmol · L-1. GhOPR3 was
overexpressed in Arabidopsis，which increased the salt tolerance and drought-resistant in Arabidopsis.
After mechanical damage，the expression level of related resistance genes and the content of endogenous
MJ were increased.

Key words: Gladiolus hybridus, JA, OPR, heterologous expression, stress resistance

中图分类号:

S 682.2+4

连青龙, 辛海波, 李晓昕, 钟雄辉, 尹义蕾, 义鸣放. 异源表达唐菖蒲GhOPR3 提高了拟南芥的抗逆性[J]. 园艺学报, 2014, 41(3): 498-508.

LIAN Qing-Long, XIN Hai-Bo, LI Xiao-Xin, ZHONG Xiong-Hui, YIN Yi-Lei, YI Ming-Fang. Heterologous Expression of Gladiolus GhOPR3 Enhances the Abiotic Stress Resistance of Arabidopsis[J]. ACTA HORTICULTURAE SINICA, 2014, 41(3): 498-508.