https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2014, Vol. 41 ›› Issue (3): 456-468.

• 蔬菜 • 上一篇    下一篇

花椰菜—黑芥渐渗系和异附加系的获得与分析

吕 晶1,2,刘 凡2,宗 梅2,韩 硕2,王幼平1,王桂香2,*   

  1. 1 扬州大学生物科学与技术学院,江苏扬州 225009;2 北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物
    生物学与种质创新重点实验室,北京 100097
  • 出版日期:2014-03-25 发布日期:2014-03-25
  • 基金资助:

    国家自然科学基金项目(31000538);北京市优秀人才培养资助培养计划项目(2012D002020000006);北京市农林科学院科
    技创新能力建设专项(KJCX201101010-19);国家‘863’计划项目(2012AA100202-3)

Identification and Characterization of Introgression and Additional Lines
Between Cauliflower and Black Mustard

Lü Jing1,2,LIU Fan2,ZONG Mei2,HAN Shuo2,WANG You-ping1,and WANG Gui-xiang2,*   

  1. 1Yangzhou University,Yangzhou 225009,China;2Beijing Vegetable Research Center,Key Laboratory of Biology and
    Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture,Beijing 100097,China
  • Online:2014-03-25 Published:2014-03-25

摘要: 以花椰菜—黑芥体细胞杂种自交及回交后代为材料,结合形态学特征,分子标记和荧光原
位杂交(FISH)等群体分析技术,在16 份高代回交及自交材料中筛选获得了花椰菜类型渐渗系材料12
份,偏花椰菜类型异附加系材料4 份。形态学调查及FISH 分析表明:12 份渐渗系材料除茎、叶等营养器
官特征与花椰菜近似外,已具有较正常的花球形成,染色体数为18 条,均来源于花椰菜;植株花粉母细
胞减数分裂行为基本正常。SSR 和AFLP 分子标记检测表明,渐渗系材料除扩增到数量不等的黑芥多态
性位点外,还扩增到埃塞俄比亚芥特异的多态性位点和少量新位点,另外发现渐渗系材料有丢失亲本花
椰菜多态性位点的现象。FISH 分析4 份异附加系材料的染色体组成,结果显示:PFCN29BC2S1-4 为9 条
花椰菜染色体附加8 条黑芥染色体;PFCN15-2S1BC5-6-2 和PFCN29BC4-37-7 为16 条花椰菜染色体分别
附加7 条和14 条黑芥染色体;PFCN29BC4-38-5 为花椰菜染色体附加了5 ~ 7 条黑芥染色体。

关键词: 花椰菜, 黑芥, 渐渗系, SSR, AFLP, FISH

Abstract: Abstract:In this research,we analyzed the asymmetrical somatic hybrids of cauliflower(Brassica
oleracea var. botrytis,CC,2n = 18)and black mustard(B. nigra,BB,2n = 16)and their selfed or
backcrossed progenies by integrating morphological,cytological and molecular information.Among 16
advanced selfing and backcrossing descendants in analysis,12 introgression lines displaying the
cauliflower-like morphology were identified with chromosome number 18. Simple sequence repeat(SSR)
and amplified fragment length polymorphism(AFLP)analysis showed that those introgression lines
preserved some DNA banding patterns from B. nigra,B. carinata(BBCC,2n = 34)and some of new
bands,and all of these introgression lines showed an absence of some cauliflower-specific sites. 4 plants
(PFCN29BC2S1-4,PFCN15-2S1BC5-6-2,PFCN29BC4-37-7 and PFCN29BC4-38-5)were confirmed to
have 9,16,16 and 18 cauliflower chromosomes added with 8,7,14 and 5–7 B. nigra-derived chromosomes respectively by fluorescence in situ hybridization(FISH).

Key words: cauliflower, Brassica nigra, introgression lines, SSR, AFLP, FISH

中图分类号: