桃3种颜色果肉中10种酚类物质的测定及比较

园艺学报 ›› 2014, Vol. 41 ›› Issue (2): 319-328.

桃3种颜色果肉中10种酚类物质的测定及比较

严娟，蔡志翔，沈志军，张斌斌，钱巍，俞明亮*

（江苏省农业科学院园艺研究所，南京 210014）

收稿日期:2013-08-16 出版日期:2014-02-25 发布日期:2014-02-25

Determination and Comparison of 10 Phenolic Compounds in Peach with Three Types of Flesh Color

YAN Juan，CAI Zhi-xiang，SHEN Zhi-jun，ZHANG Bin-bin，QIAN Wei，and YU Ming-liang*

（Institute of Horticulture，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China）

Received:2013-08-16 Online:2014-02-25 Published:2014-02-25

摘要/Abstract

摘要： 优化了桃酚类物质分析的高效液相色谱法（HPLC），并测定和比较了红、黄、白色类型桃果肉酚类物质组分。以甲醇（0.1% H₃PO₄）为提取剂，采用C₁₈柱（4.6 mm × 250 mm，5 μm），以甲醇（0.1% H₃PO₄）和水（0.1% H₃PO₄）为流动相梯度冲洗，流速1.0 mL · min^-1，温度30 ℃，检测波长280 nm，适于桃果肉酚类组分分析。在桃果肉中检出了绿原酸、新绿原酸、儿茶素、表儿茶素、芦丁、槲皮素、没食子酸、阿魏酸、根皮苷和根皮素。不同肉色类型桃的主要酚类组成和含量存在差异，红肉桃主要成分为表儿茶素、绿原酸、儿茶素和新绿原酸，黄肉桃为新绿原酸、绿原酸和儿茶素，白肉桃为新绿原酸、儿茶素和芦丁；红肉桃含量最高的酚类为表儿茶素最高（78.91 ~ 673.90 mg · kg^-1FW），黄肉桃为新绿原酸（7.28 ~ 25.57 mg · kg^-1FW），白肉桃中含量最高的酚类因品种而异，规律不明显，以新绿原酸（3.17 ~ 6.16 mg · kg^-1FW）和儿茶素（4.21 ~ 14.55 mg · kg^-1FW）较高；各酚类含量差异显著，对于绿原酸、新绿原酸、儿茶素、表儿茶素和芦丁等桃果肉中的主要组分而言，红肉桃的含量均显著高于白肉和黄肉桃。同一肉色不同品种间一些酚类物质的含量差异显著，可用于筛选桃特异种质。

关键词: 桃, 肉色, 酚类物质, 高效液相色谱法

Abstract: In this study，we optimized the High-performance liquid chromatography（HPLC）determination system for phenolic compounds in peach，and determined and compared phenolic compounds in three types of color peach flesh，i.e. blood，yellow and white. The phenolic compounds of peach flesh were extracted with ethanol（0.1% H3PO4）via ZORBAX SB-C18 column（4.6 mm × 250 mm，5 μm），by using ethanol（0.1% H3PO4）and water（0.1% H3PO4）as solvents at the flow rate of 1.0 mL · min-1 under the temperature of 30 ℃ and UV of 280 nm. This method was particularly suitable for the determination of phenolic compounds of peach flesh. The results showed that chlorogenic acid，neochlorogenic acid，catechin，epicatechin，rutin，quercetin，gallic acid，ferulic acid，phlorizin and phloretin were detected in peach flesh. The main components and content of phenolic compounds were different in the three types of flesh color peaches. In details，epicatechin，chlorogenic acid，catechin and neochlorogenic acid were the main components in blood flesh peach. Neochlorogenic acid，chlorogenic acid and catechin were the first most components in yellow flesh peach，while neochlorogenic acid，catechin and rutin in white flesh peach. Epicatechin and neochlorogenic acid were the highest phenolic compounds in blood flesh peach and yellow flesh peach，respectively，with the content of 78.91–673.90 mg · kg-1FW and 7.28–25.57 mg · kg-1FW. However，the phenolic compounds in white flesh peach was distinct among cultivars，and neochlorogenic acid（3.17–6.16 mg · kg-1FW）and catechin（4.21–14.55 mg · kg-1FW）contents were higher than the other components. In particular，the contents of phenolic compounds varied significantly in different color flesh peaches. As the main phenolic compounds in peach flesh，chlorogenic acid，neochlorogenic acid，catechin，epicatechin and rutin were significant higher in blood flesh peach than that of white and yellow flesh peach. In addition，the content of some phenolic compounds was significantly different among cultivars within the same color flesh，which may be favorably used to screen specific germplasms in peach.

Key words: peach, flesh color, phenolic compounds, high-performance liquid chromatography

中图分类号:

S 662.1

严娟, 蔡志翔, 沈志军, 张斌斌, 钱巍, 俞明亮. 桃3种颜色果肉中10种酚类物质的测定及比较[J]. 园艺学报, 2014, 41(2): 319-328.

YAN Juan, CAI Zhi-Xiang, SHEN Zhi-Jun, ZHANG Bin-Bin, QIAN Wei, YU Ming-Liang. Determination and Comparison of 10 Phenolic Compounds in Peach with Three Types of Flesh Color[J]. ACTA HORTICULTURAE SINICA, 2014, 41(2): 319-328.

[1]	翟含含, 翟宇杰, 田义, 张叶, 杨丽, 温陟良, 陈海江. 桃SAUR家族基因分析及PpSAUR5功能鉴定[J]. 园艺学报, 2023, 50(1): 1-14.
[2]	袁馨, 徐云鹤, 张雨培, 单楠, 陈楚英, 万春鹏, 开文斌, 翟夏琬, 陈金印, 甘增宇. 猕猴桃后熟过程中ABA响应结合因子AcAREB1调控AcGH3.1的表达[J]. 园艺学报, 2023, 50(1): 53-64.
[3]	邢柱东, 吕福堂, 郭尚敬, 张演义. 新品种‘聊大红金’桃[J]. 园艺学报, 2023, 50(1): 225-226.
[4]	杨兴旺, 王海波, 王莹莹, 王小龙, 王志强, 刘培培, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农甘爽’[J]. 园艺学报, 2022, 49(S2): 15-16.
[5]	杨兴旺, 王海波, 王莹莹, 张艺灿, 王宝亮, 刘培培, 史祥宾, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农白干’[J]. 园艺学报, 2022, 49(S2): 17-18.
[6]	杨兴旺, 刘凤之, 王海波, 王莹莹, 王志强, 史祥宾, 冀晓昊, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农寒水蜜’[J]. 园艺学报, 2022, 49(S2): 19-20.
[7]	杨兴旺, 刘凤之, 王海波, 王莹莹, 张艺灿, 李鹏, 王小龙, 刘万春, 王孝娣. 晚熟抗寒桃新品种‘中农秋香’[J]. 园艺学报, 2022, 49(S2): 21-22.
[8]	王莹莹, 刘立常, 刘志伍, 杨兴旺, 刘万春, 王孝娣, . 极晚熟桃新品种‘中农冬蜜’[J]. 园艺学报, 2022, 49(S2): 23-24.
[9]	王莹莹, 刘立常, 刘志伍, 杨兴旺, 刘万春, 王孝娣, . 小果油桃新品种‘中农珍珠’[J]. 园艺学报, 2022, 49(S2): 25-26.
[10]	吴延军, 刘庆忠, 陈鸿才, 戚行江, 朱东姿, 郑家祥, 曹学敏, 方丹燕. 甜樱桃新品种‘江南锦’[J]. 园艺学报, 2022, 49(S2): 29-30.
[11]	张晓明, 闫国华, 周宇, 王晶, 段续伟, 吴传宝, 张开春. 甜樱桃砧木新品种‘京春2号’[J]. 园艺学报, 2022, 49(S2): 31-32.
[12]	宋放, 陈奇, 袁炎良, 陈沙, 尹海军, 蒋迎春, . 黄肉猕猴桃新品种‘先沃1号’[J]. 园艺学报, 2022, 49(S2): 47-48.
[13]	齐永杰, 高正辉, 马娜, 王清明, 柯凡君, 陈钱, 徐义流, . 黄肉抗溃疡病猕猴桃新品种‘皖农金果’[J]. 园艺学报, 2022, 49(S2): 49-50.
[14]	张慧琴, 楼国荣, 陆玲鸿, 古咸彬, 宋根华, 谢鸣. 黄肉中华猕猴桃新品种‘金义’[J]. 园艺学报, 2022, 49(S2): 51-52.
[15]	季琳琳, 陈素传, 吴志辉, 常君, 韩文妍, 陶汝鹏. 早花山核桃新品种‘宁国山核桃2号’[J]. 园艺学报, 2022, 49(S2): 53-54.