外源NO对高温胁迫下姜叶片活性氧代谢的影响

园艺学报 ›› 2014, Vol. 41 ›› Issue (2): 277-284.

外源NO对高温胁迫下姜叶片活性氧代谢的影响

李秀，巩彪，徐坤*

（山东农业大学园艺科学与工程学院，作物生物学国家重点实验室，农业部黄淮地区园艺作物生物学与种质创制重点实验室，山东泰安 271018）

收稿日期:2013-08-21 出版日期:2014-02-25 发布日期:2014-02-25

Effects of Exogenous Nitric Oxide on Reactive Oxygen Metabolism in Ginger Leaves Under Heat Stress

LI Xiu，GONG Biao，and XU Kun*

（College of Horticulture Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology，Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region，Ministry of Agriculture，Tai’an，Shandong 271018，China）

Received:2013-08-21 Online:2014-02-25 Published:2014-02-25

摘要/Abstract

摘要： 为探讨姜对高温胁迫的生理响应特性，采用砂培法，研究了高温对姜叶片活性氧代谢的影响及培养液添加NO供体硝普钠（SNP）缓解高温胁迫的作用。结果表明，高温胁迫显著降低姜叶片色素含量，增加产生速率及H₂O₂和MDA含量；叶片SOD、POD、CAT等抗氧化酶活性在高温胁迫初期增强，随胁迫时间延长急剧下降；同时，AsA–GSH循环遭受破坏。外源添加0.1 mmol · L^-1 SNP能显著增强姜叶片的抗氧化酶活性，降低叶片ROS水平，减轻膜脂过氧化程度，维持AsA–GSH循环系统的稳定性；降低叶片色素降解，有效缓解高温对姜叶片造成的伤害，增强其耐热性。

关键词: 姜, 高温胁迫, 一氧化氮, 活性氧代谢

Abstract: To investigate the physiological response characteristics of ginger under heat stress，a sandy-cultured experiment was conducted to study the effects of high temperature on active oxygen metabolism of ginger leaves and the mitigation effect of SNP（sodium nitroprusside，a NO donor）application in ginger leaves under heat stress. Such as pigments content，reactive oxygen species level，enzymatic and non-enzymatic antioxidant systems were considered in this study. A significant decrease of pigments content as well as accumulation of ，H₂O₂ and MDA were observed in heat-stressed ginger leaves. Additionally，SOD，POD and CAT activities were enhanced at the early stage of heat stress，which were then sharply inhibited with the stress time prolonging and blocked AsA–GSH cycle. Compared with the control treatment，application of 0.1 mmol · L^-1 SNP significantly enhanced activities of antioxidant enzymes as well as palingenesis of AsA–GSH cycle that significantly decreased levels of reactive oxygen species and membrane lipid peroxidation. Furthermore，application of SNP conferred ginger plants heat stress tolerance by pigment contents analysis.

Key words: ginger, Zingiber officinale, heat stress, nitric oxide, reactive oxygen metabolism

中图分类号:

S 632.5

李秀，巩彪，徐坤*. 外源NO对高温胁迫下姜叶片活性氧代谢的影响[J]. 园艺学报, 2014, 41(2): 277-284.

LI Xiu，GONG Biao，and XU Kun*. Effects of Exogenous Nitric Oxide on Reactive Oxygen Metabolism in Ginger Leaves Under Heat Stress[J]. ACTA HORTICULTURAE SINICA, 2014, 41(2): 277-284.

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