关键词: 月季；RhPIP1, 1；启动子活性；激素；非生物胁迫

Abstract: A 2 126 bp sequence of the RhPIP1;1 promoter was isolated from rose（Rosa hybrida L.
‘Samantha’）through inverse PCR. PLACE analysis indicated that the RhPIP1;1 promoter harbors
hormone-responsive elements，such as GA，ABA，and ethylene related cis-elements，and abiotic
stresses-responsive elements，such as dehydration-，salt-，and cold-related cis-elements. In
RhPIP1;1promoter：：GUS-expressing Arabidopsis，the activity of RhPIP1;1 promoter was found to be
associated with the development process. Almost all the organs exhibited the promoter activity，which is
particularly active in expanding organs and vascular tissues of leaves and flowers. Moreover，in the 6- or
9-day-old transgenic plants，the promoter activity was up-regulated by GA treatment in the rosettes，
whereas down-regulated by ABA，mannitol，NaCl，and cold treatment in the rosettes and roots. A series of 5′ deletion fragments of the RhPIP1;1 promoter were fused with GUS gene，and transiently expressed in
tobacco leaves. It was found that deleting 324 bp fragment from–580 to–256 led to a significant
decrease of the promoter activity. Taken together，our results demonstrated that the RhPIP1;1 promoter
responds to various hormones and abiotic stresses in a developmental- and spatial-dependent manner. And
the–580 to–256 region is important for the activity of the RhPIP1;1 promoter.

Key words: rose；RhPIP1, 1；promoter activity；hormone and abiotic stress