https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2014, Vol. 41 ›› Issue (1): 80-88.

• 蔬菜 • 上一篇    下一篇

辣椒CaSn 基因超表达增强转基因烟草对南方根结线虫的抗性

张文玥1,茆振川1,*,沈宝明1,2,王 刚1,姚玉荣1,冯东昕1,谢丙炎1   

  1. 1 中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,北京 100081;2 湖南农业大学
    植物保护学院,长沙 410007
  • 出版日期:2014-01-25 发布日期:2014-01-25
  • 基金资助:

    国家重点基础研究发展计划项目(2009CB119000);国家公益性行业(农业)科研专项(201103018);国家自然科学基金项
    目(30971905);国家现代农业产业技术体系建设专项资金项目(CARS-25-B01)

Overexpression of Pepper Casn Gene Enhances Resistance to Meloidogyne incognita in Transgenic Plants

ZHANG Wen-yue1,MAO Zhen-chuan1,*,SHEN Bao-ming1,2,WANG Gang1,YAO Yu-rong1,FENG
Dong-xin1,and XIE Bing-yan1   

  1. 1Key Laboratory of Horticultural Crops Biology and Genetic Improvement,Ministry of Agriculture,Institute of Vegetables
    and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China; 2 Hunan Agricultural University,Changsha
    410007,China
  • Online:2014-01-25 Published:2014-01-25

摘要: CaSn 基因是在辣椒(Capsicum annuum)‘Santaka’中发现的新型抗菌肽Snakin 家族基因。
通过RT-PCR,将CaSn 基因从辣椒中分离并构建到pBI-121 植物表达载体,通过农杆菌EHA105 介导转
化白肋烟(Nicotiana tabacum‘White burley’),筛选到10 个独立的转基因株系,并对T1 代植株进行抗
南方根结线虫(Meloidogyne incognita)鉴定及靶标基因表达分析。结果表明CaSn 已转入白肋烟,能够进
行超量表达,并且转CaSn 基因白肋烟上的根结数量比转空载体对照和非转基因对照减少70%,同时不同
转基因烟草株系间对南方根结线虫的抗性也存在着一定差别。通过试验证明了辣椒中的抗菌肽基因CaSn
具有抗南方根结线虫作用,对于植物抗线虫资源的挖掘及利用提供了重要理论依据。

关键词: Snakin, 转基因超表达, 南方根结线虫, 抗性鉴定

Abstract: The CaSn gene is a small cysteine-rich antimicrobial peptide encoding gene and belongs to
the snakin family. The gene was cloned from pepper(Capsicum annuum‘Santaka’)by RT-PCR and
constructed to the plant expression vector pBI-121,and transformed into burley tobacco via agrobacterium
mediated method. The CaSn transgenic plants were selected by PCR and GUS staining analyses,and ten
independent transgenic tobacco plants had been obtained. The CaSn gene was stably inherited and
overexpressed in transgenic T1 generation plants by RT-qPCR detection. Compared to the empty vector
control and non-transgenetic control plants,the number of the root-knots in the transgenic plants was
reduced by 70%. Meanwhile,difference was also shown in 10 independent transgenic lines in terms of their resistance to root-knot nematodes. This study indicated that the CaSn participated in defense against
root-knot nematodes,and had an important role in excavating and utilization of nematodes-resistant
germplasms of plants.

Key words: Snakin, transgenic overexpression, Meloidogyne incognita, resistance identification

中图分类号: