https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2014, Vol. 41 ›› Issue (1): 63-72.

• 蔬菜 • 上一篇    下一篇

黄瓜种子大小遗传分析与QTL 定位

王 敏,苗 晗,张圣平,刘书林,董邵云,王 烨,顾兴芳*   

  1. 中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2014-01-25 发布日期:2014-01-25
  • 基金资助:

    国家现代农业产业技术体系建设专项资金项目(CARS-25);国家重点基础研究发展计划(‘973’)项目(2012CB113900);
    农业部园艺作物生物学与种质创制重点实验室项目

Inheritance Analysis and QTL Mapping of Cucumber Seed Size

WANG Min,MIAO Han,ZHANG Sheng-ping,LIU Shu-lin,DONG Shao-yun,WANG Ye,and GU Xing-fang*   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2014-01-25 Published:2014-01-25

摘要: 以种子大小差异显著的黄瓜野生变种‘PI183967’(Cucumis sativus var. hardwickii)和栽培
品种‘新泰密刺’选系‘931’为亲本,通过6 世代混合模型分析方法,研究种子长度和宽度的遗传规律,
并利用包含160 个株系的F9 代重组自交系群体,构建SSR 分子遗传图谱,完成种子长度、宽度及百粒质
量的QTL 染色体定位。结果表明:(1)种子长度与宽度在正、反交6 世代群体中均符合C–0 遗传模型
(加性—显性—上位性多基因遗传模型),且以多基因加性效应为主。(2)构建的永久SSR 遗传图谱,包
含149 个SSR 标记,9 个连锁群,覆盖基因组长度389.2 cM,平均标记间距为2.61 cM。(3)2012 年春季
与2013 年春季共检测到14 个与种子长度、宽度和百粒质量相关的QTL,分布在Chr.2、Chr.3、Chr.4、
Chr.5、Chr.6 染色体上,LOD 值介于2.59 ~ 9.39 之间,可解释7.4% ~ 28.3%的表型变异率,贡献率 ≥ 10.0%
的QTL 位点6 个,占QTL 总数的42.9 %,可在两年中重复检测出的QTL 位点3 个,占QTL 总数的21.5%。

关键词: 黄瓜, 种子大小, 遗传分析, QTL 定位

Abstract: The cucumber wild variety‘PI183967’(Cucumis sativus var. hardwickii)and a cultivated
cucumber Northern Chinese inbred line‘931’were used as parental materials to construct genetic
populations because of their significant difference in seed size. The inheritance of seed length and width
was studied by joint segregation analysis using positive and negative six-generation populations. A SSR
genetic map was constructed using the RILs to finish QTL mapping of seed length,width and 100-seed
weight. Results showed that(1)The inheritance of seed length and width fitted into C–0 model(Additive
dominance-epistasis polygene inheritance model)in the positive and negative cross six-generation,and
mainly with additive effect of multi-genes(2)A SSR linkage map of cucumber was constructed 149 SSR
markers. The map included 9 linkages and spanned 389.2 cM with a mean marker interval of 2.61 cM.(3)
14 QTLs related to seed length,seed width and 100-seed weight were detected 2012 and 2013 spring.
These QTLs accounted for 7.4%–28.3% of phenotypic variance were located on Chr.2,Chr.3,Chr.4,Chr.5,Chr.6 with LOD scores of 2.59–9.39. Of all the QTLs,6 QTLs explained over 10.0% of phenotypic
variance accounting for 42.9% and 3 QTLs could be detected in different years which accounted for
21.5%.

Key words: cucumber, seed size, genetic analysis, QTL mapping

中图分类号: