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园艺学报 ›› 2013, Vol. 40 ›› Issue (12): 2505-2512.

• 研究报告 • 上一篇    下一篇

硫对大葱生长及氮硫同化关键酶活性的影响

孔灵君, 徐 坤, 张永征, 何平   

  1. (山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,农业部黄淮地区园艺作物生物学与种质创制重点实验室,山东泰安 271018)
  • 出版日期:2013-12-25 发布日期:2013-12-25

Effects of Sulfur on Growth and Key Enzyme Activities Involved in Nitrogen and Sulfur Assimilation in Chinese Spring Onion

KONG  Ling-Jun, XU   Kun, ZHANG  Yong-Zheng, HE  Ping   

  1. (State Key Laboratory of Crop Biology/Ministry of Agriculture Key Laboratory of Biology and Genetic Improvement of
    Horticultural Crops in Huang-Huai Region/College of Horticulture Science and Engineering,Shandong Agricultural
    University,Tai’an,Shandong 271018,China)
  • Online:2013-12-25 Published:2013-12-25

摘要: 为探讨硫对大葱(Allium fistulosum L. var. giganteam Makino)生长及氮硫吸收同化的影响,
采用盆栽沙培方法,研究了0.01、2.01、4.01 和6.01 mmol · L-1 不同供硫水平下‘天光’大葱的生长量、
植株氮硫含量及氮硫同化关键酶活性的变化。结果表明,适当提高供硫水平,可显著促进大葱生长,提
高植株氮、硫含量及产量和品质,硫水平过高,则大葱产量降低,品质下降。大葱叶片硫酸化酶(ATPS)
活性随硫水平的升高而降低,而乙酰丝氨酸水解酶(OASS)、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)
活性在不同生长期的表现不尽相同,大葱生长前期,较高硫水平提高了NR 活性,但对GS 和OASS 活性
无显著影响;大葱生长中后期,适量提高供硫水平显著增强了OASS、GS 活性,但降低了NR 活性,表
明供硫水平可显著影响大葱对氮硫的同化利用。综合分析表明,大葱营养液硫水平以2.01 mmol · L-1 较佳,
其产量较对照0.01 mmol · L-1 增加19.13%,且综合品质显著改善。

关键词: 大葱, 硫, 产量, 品质, 氮硫同化

Abstract: In order to investigate the effects of sulfur on the growth,the nitrogen and sulfur
assimilation of Chinese spring onion,the plant growth,nitrogen and sulfur content and activity of key
enzyme(NR,GS,ATPS,OASS)for assimilation of nitrogen and sulfur in the different S levels were
studied with potted sandy culture. The results showed that the growth,the N and S content,the yield and
quality were improved by appropriately increasing the content of sulfur,while the yield and quality were
reduced when the content of S level was over 4.01 mmol · L-1. The activity of ATP sulfurylase(ATPS)of
leaves reduced with the S level increasing,while the activities of O-acetylserine thiollyase(OASS),nitrate
reductase(NR)and glutamine synthetase(GS)responded differently at each growth stage. In the initial
stage,the activity of NR was improved on the higher S level which had no significant effect on the
activities of GS and OASS. In the later stage,the activities of OASS and GS were enhanced on the appropriate S level,and the activity of NR was reduced. Comprehensive analysis of results showed that,
the nutrient solution containing 2.01 mmol · L-1 was the optimal to improving the yield and quality of
Chinese spring onion,and the yield was 19.1% higher than the control(0.01 mmol · L-1).

Key words: Chinese spring onion, sulfur, yield, quality, nitrogen and sulfur assimilation

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