关键词: 新疆野生樱桃李, 根结线虫, psoRPM1 基因, 转基因

Abstract: The root-knot nematode，especially Meloidogyne incongnita is one of the most harmful
pests could induce many crop diseases. Xinjiang wild cherry plum（Prunus sogdiana）is a kind of wild and
endemic specie in China with significant resistant effect against M. incognita. Here we isolated a gene
from Xinjiang wild cherry plum，which named psoRPM1，having responses to the infection of M.
incognitain the resistant plant. Using Agrobacterium-mediated transgenic technology，psoRPM1 gene was
introduced into tobacco varieties‘W38’，which is highly susceptible to M. incognita. Then the transgenic
tobaccos and plants of control group were inoculated with M. incognita. After growing for 60 d，the
psoRPM1-W38 showed varying degrees of resistance to M. incognita，even some of the plants nearly had
no root-knots. Comparing to the tobaccos of the control group，it is suggested that psoRPM1 gene is
capable of helping the tobacco‘W38’set up resistance mechanism against the infection of M. incognita
and finally predict the psoRPM1 is a new root-knot nematode resistance gene candidate.

Key words: Prunus sogdiana, root-knot nematode, psoRPM1 gene, transgenic