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园艺学报 ›› 2013, Vol. 40 ›› Issue (12): 2453-2462.

• 蔬菜 • 上一篇    下一篇

胡萝卜FLC 同源基因对低温及光周期响应

 毛笈华, 庄飞云, 欧承刚, 赵志伟, 王 慧, 马振国   

  1. (中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,北京 100081)
  • 出版日期:2013-12-25 发布日期:2013-12-25

Expression Analysis of FLC Homologues Responding to Low Temperature and Photoperiod in Carrot

 MAO  Ji-Hua, ZHUANG  Fei-Yun,OU Cheng-Gang , ZHAO  Zhi-Wei, WANG   Hui, MA  Zhen-Guo   

  1. (Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
  • Online:2013-12-25 Published:2013-12-25

摘要: 基于‘松滋野生’(Ws)和‘Amsterdam’(Af)胡萝卜转录组测序,挖掘胡萝卜FLC 同源
基因(DcFLCs),深入研究其对低温及光周期响应规律。结果表明,胡萝卜中存在3 个具有完整MADS-box
和K-box 保守结构域的FLC 同源基因DcFLC1、DcFLC2、DcFLC3,分别编码209、212、219 个氨基酸
残基蛋白,进化树显示与其它植物FLC 亲缘关系较远。RT-PCR 表明DcFLC1 和DcFLC2 在供试材料中均
表达,而DcFLC3 仅在部分材料中表达。qPCR 结果显示低温能促进DcFLC1 和DcFLC3 在耐抽薹品种
Af 幼苗叶片和种根中表达,而DcFLC2 仅在Af 种根中表达显著,在幼苗叶片中表达不显著。DcFLC1 和
DcFLC2 在抽薹敏感的Ws 幼苗叶片和种根春化过程中表达规律不同,低温能促进DcFLC1 在幼苗叶片以
及DcFLC2 在种根中表达。连续光照能促进DcFLC1、DcFLC2 在Af 幼苗叶片中表达,但在Ws 中则不同。

关键词: 胡萝卜, DcFLCs, 低温, 光周期, 基因表达

Abstract: Carrot(Daucus carota L.)is a biennial species and requires vernalization for flowering.
The premature bolting of carrot occurs in winter-spring plastic tunnel and spring cultivation,and results in
a complete loss of commercial value,but limited progress reported on the control of bolting and flowering
in carrot. Basis on the transcriptome sequences,carrot FLOWERING LOCUS C homologues(DcFLCs)
were screened. Cultivar‘Amsterdam’(Af)tolerance to premature bolting and wild sensitive species
‘Songzi’(Ws)were selected to study the relative expression of DcFLCs under cold and photoperiod
treatment examined by real-time qPCR. Three FLC homologues(DcFLC1,DcFLC2 and DcFLC3)were
annotated with complete ORFs,MADS-box and K-box conserved regions,which encoded 209,212 and
219 amino acids,respectively. Phylogenetic analysis showed that DcFLCs were clustered with other plants
FLC homologous. DcFLC1 and DcFLC2 were confirmed to express in all species by RT-PCR,but
DcFLC3 only in some. The relative expression of DcFLC1 and DcFLC3 were improved in Af young plants
and roots during vernalization,while DcFLC2 was only improved in roots but no significance in young plants. DcFLC1 were improved in Ws young plants but no significance in roots during vernalization,while
DcFLC2 in contrary. Both of the relative expression of DcFLC1 and DcFLC2 were improved in Af young
plants under continuous illumination,but different in Ws.

Key words: carrot, DcFLCs, low temperature, photoperiod, gene expression

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