关键词: 荔枝, 果皮衰老, 抑制差减杂交, cDNA 微阵列, 基因克隆

Abstract: The differentially expressed genes between pericarp of‘Feizixiao’fruit at 0 h and 32 h after
harvest（HAH）were identified by the combination of suppression subtractive hybridization（SSH）and
cDNA microarray technology. The reciprocal，forward and reverse，SSH libraries were constructed with
RNA extracted from the pericarp at 0 HAH and 32 HAH as tester and driver，respectively. Then 282 and
76 positive clones were obtained from the forward and reverse library. However，only 17 and 49 clones
with enhanced or repressed expression at 32 HAH were identified by cDNA microarray hybridization，
representing 16 and 17 genes both with enhanced and repressed expression in the pericarp at 32 HAH，
respectively. Among the tested genes，genes encoding enzymes involved in carbohydrate metabolism，cell
wall metabolism and genes encoding heat shock proteins had higher frequencies than other genes. The
results of cDNA microarray hybridization were confirmed by RT-PCR.

Key words: litchi, pericarp browning, suppression subtractive hybridization, cDNA microarray, gene cloning