关键词: 香蕉, ERF基因, 克隆, 表达分析

Abstract: A full length cDNA named MaERF which was cloned from banana on the basis of a AP2/ERF fragment combined with RACE technology. The full cDNA of this gene was 1 611 bp，the open reading frame was 1 128 bp and coded a polypeptide of 376 amino acids. This gene had one typical AP2/ERF domain. The genome sequence full-length of this gene was 2 881 bp，and contained one intron splice site according to the rules of the“GT-AG”. The phylogenetic tree shows that the gene belongs to B2 subsets of ERF genes，and they are more involved in plant stress reactions. Semi-quantitative RT-PCR analysis showed that the gene was expressed in various organs of the banana，with lower the amount of expression in the roots and leaves，while high expression in fruit and flowers；Fluorescence quantitative real-time PCR detection showed this gene had high expression with Fusarium oxysporum and low temperature stress，so this gene may play an important role in bananas stress reaction.

Key words: banana, ERF gene；clone, expression analysis