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园艺学报 ›› 2013, Vol. 40 ›› Issue (8): 1535-1540.

• 其它园艺植物 • 上一篇    下一篇

低温对茶树花粉管抑制作用与NO关系的研究

王伟东1,蒋 芯1,杜昱林1,王玉花1,2,*,黎星辉1,2,*   

  1. (1南京农业大学茶叶科学研究所,南京 210095;2江苏省茶学博士后科研工作站,江苏苏州 215128)
  • 收稿日期:2013-03-28 出版日期:2013-08-25 发布日期:2013-08-25

A Study on the Involvement of Nitric Oxide in Inhibiting Pollen Tube Growth of Tea Plant(Camellia sinensis)Under Low-temperature

WANG Wei-dong1,JIANG Xin1,DU Yu-lin1,WANG Yu-hua1,2,*,and LI Xing-hui1,2,*   

  1. (1Tea Research Institute,Nanjing Agricultural University,Nanjing 210095,China; 2The Post-Doctoral Research Station for Tea Science of Jiangsu Province,Suzhou,Jiangsu 215128,China)
  • Received:2013-03-28 Online:2013-08-25 Published:2013-08-25

摘要: 以无性系茶树品种‘龙井43’花粉为材料,研究了低温对茶树花粉萌发和花粉管伸长的影响及其与一氧化氮(NO)的关系。结果显示低温显著抑制了茶树花粉萌发和花粉管伸长,且表现出明显的时间依赖性;外源NO供体硝普钠(SNP)产生类似的抑制效应,并表现出明显的浓度依赖性,其抑制效应被NO清除剂(cPTIO)和一氧化氮合酶(NOS)抑制剂(L-NNA)所缓解。此外,低温处理促进茶树花粉管NO释放并伴随着NOS活性的提高。上述研究结果表明,低温抑制茶树花粉萌发和花粉管伸长与其诱导NO释放有关,且低温诱导的NO释放部分依赖于NOS催化产生。

关键词: 茶树, 低温, 花粉萌发, 花粉管伸长, 一氧化氮

Abstract: The pollen germination and pollen tube growth of tea plant,as well as their relationship with nitric oxide(NO)under low-temperature were studied using pollen of culivar‘Longjing 43’. The results showed that low-temperature significantly inhibited the tea pollen germination and pollen tube growth in a time-dependent manner. Similar inhibitory effects in a dose-dependent manner were observed with exogenous NO donor(Sodium Nitroprusside,SNP). Both NO scavenger(cPTIO)and nitric oxide synthase(NOS)inhibitor(L-NNA)partially alleviated this inhibitory effect. Furthermore,the NO releasing along with the NOS activity were promoted in tea pollen tubes under low-temperature. The results indicated that the inhibitory effects of low-temperature treatment on tea pollen germination and pollen tube growth are related to NO generating from NOS activity.

Key words: tea plant, low-temperature, pollen germination, pollen tube growth, nitric oxide