外源海藻糖对高温胁迫下肺形侧耳氧化损伤的缓解效应

园艺学报 ›› 2013, Vol. 40 ›› Issue (8): 1501-1508.

外源海藻糖对高温胁迫下肺形侧耳氧化损伤的缓解效应

刘秀明，黄晨阳，陈强，邬向丽，张金霞*

（中国农业科学院农业资源与农业区划研究所，农业部农业微生物资源收集与保藏重点实验室，北京 100081）

收稿日期:2013-05-03 出版日期:2013-08-25 发布日期:2013-08-25

Alleviative Effects of Exogenous Trehalose on Oxidative Damage Metabolism in Pleurotus pulmonarius Under Heat Stress

LIU Xiu-ming，HUANG Chen-yang，CHEN Qiang，WU Xiang-li，and ZHANG Jin-xia*

（Institute of Agricultural Resources and Regional Planning，Chinese Academy of Agricultural Sciences/Key Laboratory of Microbial Resources Collection and Preservation，Ministry of Agriculture，Beijing 100081，China）

Received:2013-05-03 Online:2013-08-25 Published:2013-08-25

摘要/Abstract

摘要： 高温胁迫影响食用菌的品质和产量，由高温引起的氧化胁迫是导致损伤的主要原因。以肺形侧耳（Pleurotus pulmonarius）热敏感菌株CCMSSC 00494和耐热菌株CCMSSC 00499为试验材料，研究了外源海藻糖对其菌丝体高温胁迫下氧化损伤的缓解效应。结果表明，高温（40 ℃）胁迫48 h，热敏感菌株中硫代巴比妥酸反应物（TBARS）含量（代表氧化损伤程度）高于耐热菌株。外源海藻糖处理可以显著降低高温胁迫下菌丝体内产生速率、H₂O₂含量、脂氧合酶（LOX）活性和TBARS含量水平，缓解高温胁迫所引发的氧化损伤。另外，外源海藻糖对超氧化物歧化酶（SOD）活性有保护和提高的作用，对过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性有抑制作用，对过氧化物酶（POD）活性影响不明显。外源海藻糖对热敏感菌株的保护效应高于耐热菌株。

关键词: 肺形侧耳, 海藻糖, 高温胁迫, 耐热性, 抗氧化酶

Abstract: Heat stress affects the quality and productivity of mushroom. Oxidative stress triggered by heat stress is the main reasons that lead to damage. We investigated the alleviative effects of exogenous trehalose on heat-induced oxidative stress in two Pleurotus pulmonarius strains，heat sensitive strain CCMSSC 00494 and heat tolerant strain CCMSSC 00499. The results showed that the TBARS level in heat sensitive strain was significantly higher than that in heat tolerant strain when heat stress（40 ℃）lasted 48 h，and the tolerance of heat-induced oxidative damage of heat tolerant strain was better than heat sensitive strain. Exogenous trehalose could alleviate the oxidative damage induced by heat stress by reducing the generation rate of and H₂O₂ and the activity of LOX and the content of TBARS. Additionally，exogenous trehalose had a positive effect on the activity of antioxidants SOD，and a negative effect on enzymetic antioxidants including CAT and APX，but had no effect on enzymetic antioxidants POD under the heat stress. Besides，the effect of exogenous trehalose on heat sensitive strain was more obvious than that of heat tolerant strain.

Key words: Pleurotus pulmonarius, trehalose, heat stress, heat resistance, enzymetic antioxidants

刘秀明，黄晨阳，陈强，邬向丽，张金霞*. 外源海藻糖对高温胁迫下肺形侧耳氧化损伤的缓解效应[J]. 园艺学报, 2013, 40(8): 1501-1508.

LIU Xiu-ming，HUANG Chen-yang，CHEN Qiang，WU Xiang-li，and ZHANG Jin-xia*. Alleviative Effects of Exogenous Trehalose on Oxidative Damage Metabolism in Pleurotus pulmonarius Under Heat Stress[J]. ACTA HORTICULTURAE SINICA, 2013, 40(8): 1501-1508.

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