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园艺学报 ›› 2013, Vol. 40 ›› Issue (8): 1494-1500.

• 蔬菜 • 上一篇    下一篇

NaHSO3对姜叶片光合作用与活性氧及氮代谢关键酶活性的影响

李海东1,张永征1,徐 坤1,*,肖 静2   

  1. (1山东农业大学园艺科学与工程学院/作物生物学国家重点实验室/农业部黄淮地区园艺作物生物学与种质创制重点实验室,山东泰安 271018;2山东省泰安市岱岳区农业局农技站,山东泰安 271000)
  • 收稿日期:2013-02-10 出版日期:2013-08-25 发布日期:2013-08-25

Effects of NaHSO3 on Photosynthesis and Reactive Oxygen and Nitrogen Metabolism Enzyme Activity in Ginger Leaves

LI Hai-dong1,ZHANG Yong-zheng1,XU Kun1,*,and XIAO Jing2   

  1. (1College of Horticulture Science and Engineering,Shandong Agricultural University/State Key Laboratory of Crop Biology/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region,Ministry of Agriculture,Tai’an,Shandong 271018,China;2Agrotechnical Station,Agricultural Bureau of Daiyue District,Tai’an,Shandong 271000,China)
  • Received:2013-02-10 Online:2013-08-25 Published:2013-08-25

摘要: 采用光呼吸抑制剂NaHSO3抑制光呼吸后,测定了姜叶片光合作用相关参数、叶绿素荧光参数、米勒反应及氮代谢关键酶活性等的变化。结果表明,NaHSO3处理姜叶片1 d后,净光合速率较对照上升了19%,但随后呈现出下降趋势,至处理后6 d时比对照降低了17%,同时伴随着GsCi的降低,NaHSO3处理显著降低了Fv/FmΦPSIIqP,提高了NPQ,增强了米勒反应,增加了过氧化氢含量,降低了硝酸还原酶、谷氨酰胺合成酶和谷氨酸合酶活性,表明NaHSO3抑制姜叶片光呼吸的同时,也显著抑制了碳氮代谢。

关键词: 姜, 光呼吸, 光能耗散, 氮代谢

Abstract: Gas exchange,chlorophyll fluorescence,the Mehler reaction,and activities of nitrate reductase,glutamine synthetase,and glutamate synthase were measured after addition of the photorespiration inhibitor NaHSO3 in ginger leaves. According to our results,after 1 day of NaHSO3 treatment,the net photosynthetic rate increased by 19% compared with the control. The rate subsequently decreased and was reduced by 17% compared with the control on day 6. Meanwhile,stomatal conductance and the intercellular CO2 concentration decreased. NaHSO3 treatment significantly decreased the Fv/FmФPSIIqP,and activities of nitrate reductase,glutamine synthetase and glutamate synthase,and increased NPQ,the Mehler reaction,and H2O2 concentration. These results show that carbon and nitrogen metabolism is reduced when NaHSO3 inhibits photorespiration in ginger leaves.

Key words: ginger, photorespiration, light energy allocation, nitrogen metabolism