大白菜pol CMS育性恢复基因的表达分析

园艺学报 ›› 2013, Vol. 40 ›› Issue (8): 1475-1486.

大白菜pol CMS育性恢复基因的表达分析

徐文玲1,2, 王淑芬2，王翠花2，刘贤娴2，付卫民2，何启伟2,*，赵双宜3,*

（1山东农业大学园艺科学与工程学院，山东泰安 271018；2山东省农业科学院蔬菜研究所，山东省设施蔬菜生物学重点试验室，国家蔬菜改良中心山东分中心，济南 250100；3山东大学生命科学学院，济南 250100）

收稿日期:2013-04-11 出版日期:2013-08-25 发布日期:2013-08-25

Gene Expression Profiling Analysis of pol CMS Fertility-restorer Genes in Chinese Cabbage（Brassica rapa L. ssp. pekinensis）

XU Wen-ling1,2，WANG Shu-fen2，WANG Cui-hua2，LIU Xian-xian2，FU Wei-min2，HE Qi-wei2,*，and ZHAO Shuang-yi3,*

（1College of Horticulture Science and Engineering，Shandong Agricultural University，Tai’an，Shandong 271018，China；2Institute of Vegetable Research of Shandong Academy of Agricultural Science，Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province，National Improvement Center for Vegetable，Shandong Branch，Ji’nan 250100，China；3School of Life Science of Shandong University，Ji’nan 250100，China）

Received:2013-04-11 Online:2013-08-25 Published:2013-08-25

摘要/Abstract

摘要： 为进一步探明pol CMS育性恢复基因作用的分子机理，利用数字基因表达谱技术，选用不育系与恢复系杂交的F₂代分离群体，对大白菜polCMS育性恢复相关基因的差异表达进行了分析，并选取部分基因进行了实时荧光定量PCR验证。共有2 826个基因差异表达，其中441个上调表达，2 385个下调表达。GO功能注释表明，差异表达基因显著富集的细胞位置为细胞质、细胞器及大分子复合物等位置，细胞器包括线粒体、叶绿体及质体等，分子功能主要为核酸外切酶的活性，参与的生物过程是花粉壁的形成和组装。与pol CMS显著相关的通路主要是核糖体、糖和氨基酸代谢、核苷酸切除和修复、RNA降解等通路。表达谱和RT-PCR结果表明恢复基因主要通过下调表达调控育性恢复，有4个差异表达基因与育性恢复密切相关。

关键词: 大白菜, pol CMS, 育性恢复, 差异表达, 实时荧光定量PCR

Abstract: To further reveal the molecular mechanism of pol CMS fertility-restorer genes，digital gene expression profiling technology was used to choose the F₂ segregating population hybrid of sterile lines and restorer lines. By using the F₂ lines，differential expressions of Chinese cabbage pol CMS fertility- restorer genes were analyzed and real-time fluorescence quantitative PCR was used to validate some genes. There were 2 826 differentially expressed genes，441 of which were up-regulated，and 2 835，down-regulated. GO functional annotations showed that many differentially expressed genes were significantly clustered in cytoplasm，organelle，and macromolecular complex etc. Organelles were mainly mitochondria，chloroplasts and plastid etc. These genes showed exonuclease activities and were. involved in the biological processes of pollen wall formation and assembly. Metabolic pathways significantly related to pol CMS were mainly the pathways of ribosomes，glucose and amino acid metabolism，peroxidase，nucleotide excision and repair，and RNA degradation. The gene expression profile and RT-PCR results indicate that restorer genes regulate fertility restoration by down-regulation. Further，four differentially expressed genes were shown to be closely related to fertility restoration were closely related to fertility restoration.

Key words: Chinese cabbage, pol CMS, fertility restorer gene, differentially expression, real-time qPCR

徐文玲1,2, 王淑芬2，王翠花2，刘贤娴2，付卫民2，何启伟2,*，赵双宜3,*. 大白菜pol CMS育性恢复基因的表达分析[J]. 园艺学报, 2013, 40(8): 1475-1486.

XU Wen-ling1,2，WANG Shu-fen2，WANG Cui-hua2，LIU Xian-xian2，FU Wei-min2，HE Qi-wei2,*，and ZHAO Shuang-yi3,*. Gene Expression Profiling Analysis of pol CMS Fertility-restorer Genes in Chinese Cabbage（Brassica rapa L. ssp. pekinensis）[J]. ACTA HORTICULTURAE SINICA, 2013, 40(8): 1475-1486.

[1]	李镇希, 潘睿翾, 许美容, 郑正, 邓晓玲. 柑橘黄龙病菌双重实时荧光PCR检测方法的建立[J]. 园艺学报, 2023, 50(1): 188-196.
[2]	韩书辉, 韩彩锋, 韩书荣, 韩彩梅, 韩旭. 秋大白菜新品种‘胶研秋宝’[J]. 园艺学报, 2022, 49(S2): 83-84.
[3]	汪维红, 张凤兰, 余阳俊, 张德双, 赵岫云, 于拴仓, 苏同兵, 李佩荣, 辛晓云. 秋大白菜新品种‘京秋1518’[J]. 园艺学报, 2022, 49(S2): 85-86.
[4]	余阳俊, 汪维红, 苏同兵, 张凤兰, 张德双, 赵岫云, 于拴仓, 李佩荣, 辛晓云, 王姣. 抗根肿病耐抽薹大白菜新品种‘京春CR3’[J]. 园艺学报, 2022, 49(S2): 87-88.
[5]	王丽丽, 王鑫, 吴海东, 温蔷, 杨晓飞. 抗根肿病大白菜新品种‘辽白28’[J]. 园艺学报, 2022, 49(S2): 89-90.
[6]	余阳俊, 苏同兵, 张凤兰, 张德双, 赵岫云, 于拴仓, 汪维红, 李佩荣, 辛晓云, 王姣, 武长见. 紫色苗用型大白菜新品种‘京研紫快菜’[J]. 园艺学报, 2022, 49(S2): 91-92.
[7]	徐立功, 韩太利, 孙继峰, 杨晓东, 谭金霞. 苗用大白菜新品种‘锦绿2号’[J]. 园艺学报, 2022, 49(S1): 67-68.
[8]	王钰, 张雪, 张学颖, 张思雨, 闻婷婷, 王迎君, 甘彩霞, 庞文星. 抗毒素Camalexin对大白菜抗根肿病的作用研究[J]. 园艺学报, 2022, 49(8): 1689-1698.
[9]	张秋悦, 刘昌来, 于晓晶, 杨甲定, 封超年. 盐胁迫条件下杜梨叶片差异表达基因qRT-PCR内参基因筛选[J]. 园艺学报, 2022, 49(7): 1557-1570.
[10]	张鲁刚, 卢倩倩, 何琼, 薛一花, 马晓敏, 马帅, 聂姗姗, 杨文静. 紫橙色大白菜新种质的创制[J]. 园艺学报, 2022, 49(7): 1582-1588.
[11]	周陈平, 杨敏, 郭金菊, 邝瑞彬, 杨护, 黄炳雄, 魏岳荣. 番木瓜成熟过程中全基因组DNA甲基化和转录组变化分析[J]. 园艺学报, 2022, 49(3): 519-532.
[12]	黄静静, 刘伟, 刘玉梅, 韩风庆, 方智远, 杨丽梅, 庄木, 张扬勇, 吕红豪, 王勇, 季家磊, 李占省. Ogura CMS青花菜育性恢复材料创制及其遗传背景研究[J]. 园艺学报, 2022, 49(3): 533-547.
[13]	王光鹏, 刘同坤, 徐新凤, 李竹帛, 高瞻远, 侯喜林. 大白菜LEA家族基因的鉴定及其部分成员在低温胁迫下的表达分析[J]. 园艺学报, 2022, 49(2): 304-318.
[14]	孟臻, 张伟萍, 王莹, 李龙, 姬小雪, 董贝, 乔康. 番茄枯萎病菌RT-PCR检测技术的建立与应用[J]. 园艺学报, 2022, 49(11): 2479-2488.
[15]	侯天泽, 易双双, 张志群, 王健, 李崇晖. 秋石斛RT-qPCR内参基因的筛选与验证[J]. 园艺学报, 2022, 49(11): 2489-2501.