https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2013, Vol. 40 ›› Issue (7): 1278-1288.

• 蔬菜 • 上一篇    下一篇

超量表达BoFLC3AtFT 对芥菜开花期和抗寒性的影响

杨海鹏,蔡一鸣,张 茜,任雪松,司 军,李成琼,宋洪元   

  1. 西南大学园艺园林学院,南方山地园艺学教育部重点实验室,重庆市蔬菜学重点实验室,重庆 400715
  • 收稿日期:2013-02-27 出版日期:2013-07-25 发布日期:2013-07-25
  • 基金资助:

    重庆市自然科学基金项目(cstc2011jjA80014);中央高校基本科研业务费项目(XDJK2009B024);国家科技支撑计划项目(2012BAD02B01-4);重庆市科技攻关项目[CSTC2012GG(8005)]

Influence of Over-expression of the BoFLC3 and AtFT Genes on Floweringtime and Cold Tolerance in Brassica juncea

 YANG  Hai-Peng, CAI  Yi-Ming, ZHANG   Qian, REN  Xue-Song, SI   Jun, LI  Cheng-Qiong, SONG  Hong-Yuan   

  1. College of Horticulture and Landscape Architecture,Southwest University;Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education;Key Laboratory of Olericulture,Chongqing 400715,China
  • Received:2013-02-27 Online:2013-07-25 Published:2013-07-25

摘要: 将来自甘蓝的BoFLC3 基因和拟南芥的AtFT 基因在芥菜中单独或共同表达发现,BoFLC3超量表达后,无论是长日照还是短日照条件下,转基因芥菜植株开花时间均明显延后;BoFLC3 超量表达植株低温春化处理后,相对于未春化处理的转基因植株出现花期提前现象,但仍晚于非转基因对照植株;AtFT 基因超量表达植株开花时间比非转基因对照植株大幅度提前。BoFLC3 植株与AtFT 植株杂交获得的BoFLC3 和AtFT 共表达植株开花时间与非转基因对照基本接近,显示BoFLC3AtFT 基因超表达对植物开花时间的影响效应可以相互抵消。同时,超量表达BoFLC3 基因的芥菜植株抗寒性大幅度提高,而AtFT基因的超量表达不影响植株抗寒性,表明BoFLC3 基因可能参与了植物的抗寒反应。

关键词: 芥菜, BoFLC3 基因, AtFT 基因, 开花时间, 抗寒性

Abstract: BoFLC3 gene from Brassica oleracea and AtFT gene from Arabidopsis were overexpressed alone or together in Brassica juncea. The results showed constitutive expression of the BoFLC3 gene in Brassica juncea significantly delayed flowering under long-day photoperiods as well as short-day photoperiods. Vernalization treatment induced earlier flowering of BoFLC3 overexpression plants than unvernalized transgenic control but still were flowering later than the wild type plants; the overexpression of the AtFT gene could induce early flowering in Brassica juncea. The flowering time of BoFLC3 and AtFT genes co-expression plants after cross were almost at the same time compared with the wild type plants,indicated the effects of the over-expression of the BoFLC3 and AtFT genes on flowering time could cancel each other out. Moreover,the cold-tolerance was enhanced by constitutive expression of the BoFLC3 gene in Brassica juncea,but no cold-tolerance changes were observed in AtFT overexpression plants. The results indicated the BoFLC3 should involve in plant cold-tolerance regulation.

Key words: Brassica juncea, BoFLC3 gene, AtFT gene, flowering time, cold-tolerance

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