关键词: 瓜叶菊, 花青素苷, 花色, 谷胱甘肽转移酶, 基因克隆, 表达分析

Abstract: We cloned 10 glutathione transferase（GST）genes from Senecio cruentus and called after ScGST1–10. ScGST3 was considered to be an alternative gene which related to the transfer of anthocyanin based on the similarity compare of sequence and analysis of gene expression. The amplified sequence of ScGST3 contains a 639 bp open reading frame，which encodes 212 amino acid residues. It also contains 3 exons and 2 introns and belongs to phi type GST. The compare analysis of amino acid sequence showed that the similarity between ScGST3 and other GSTs that related to the transfer of anthocyanin（such as DcGSTF2 in Dianthus caryophyllus，CkmGST3 in Cyclamen persicum and PhAN9 in Petunia hybrida and so on）were higher. The analysis of fluorescent quantitation PCR showed that this gene had a higher expression in the tissues which contain anthocyanin. The expression reached the highest abundance and decreased at the early and final stages of inflorescence development，respectively. In conclusion，we inferred that ScGST3 might associate with the transfer and accumulation of anthocyanin in Senecio cruentus.

Key words: Senecio cruentus, anthocyanin, flower color, glutathione S-transferase, gene clone, expression analysis