关键词: 黄瓜, CsARF10, 基因克隆, 序列分析, 表达分析

Abstract: Three ARF10 genes（Auxin-Respond-Factor）named CsARF10a，CsARF10b and CsARF10c，were screened from the cucumber genome database by protein homology BLAST and cloned from 8419s-1 by polymerase chain reaction（PCR）. In present study，phylogenetic reconstruction indicated that the CsARF10a has closer relation with SlARF10，while CsARF10b and CsARF10c have higher similarity with AtARF10. Promoter prediction showed that the promoters consist of diversified multiple hormone response elements. The real-time quantitative PCR（RT-qPCR）analysis proved that the expression of cucumber ARF10 genes could be regulated by different phytohormones or concentrations of 1-naphthylacetic acid（NAA）and performed different expression patterns. It was also seen that CsARF10 gene subfamily had high transcriptional levels either in roots，stems，leaves or in male flowers；In additional，the qPCR results showed that although the expression of CsARF10 genes have similar transcriptional trend，the CsARF10a had high expression level while mRNA of CsARF10b and CsARF10c were relatively low.

Key words: cucumber, CsARF10, gene cloning, homologous analysis, expression analysis