半矮生型桃节间长度相关基因的筛选和鉴定

园艺学报 ›› 2013, Vol. 40 ›› Issue (5): 943-.

半矮生型桃节间长度相关基因的筛选和鉴定

鲁振华, 李钰婷, 王志强*, 牛良, 崔国朝, 王玉兰

（中国农业科学院郑州果树研究所，国家桃葡萄品种改良中心，农业部果树育种技术重点实验室，郑州 450009）

出版日期:2013-05-25 发布日期:2013-05-25

Screening and Identification of Genes Involved in Regulating Internode Length for Semi-dwarf Peach

LU Zhen-Hua, LI Yu-Ting, WANG Zhi-Qiang-*, NIU Liang, CUI Guo-Chao, WANG Yu-Lan

（Zhengzhou Fruit Research Institute，Chinese Academy of Agricultural Sciences，National Peach and Grape Improvement
Center，Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture，Zhengzhou 450009，China）

Online:2013-05-25 Published:2013-05-25

摘要/Abstract

摘要： 为了进一步研究调控节间长度相关基因的表达模式及为克隆该半矮生性状的基因提供依据，
采用cDNA-AFLP 分析方法对半矮生型桃节间“生长跃变期”差异表达的基因进行筛选，并对差异的转录
衍生片段（TDFs）进行回收、克隆、测序、功能注释及qRT-PCR 验证。采用256 对引物，共产生9 021
个TDFs，有明显差异的TDFs 共899 个，成功回收到497 个TDFs，254 条序列与已知基因高度相似。差
异表达的基因主要涉及到信号转导、生长发育、转录调控、蛋白质代谢、防御和物质与能量代谢等。选
取其中26 个基因进行实时荧光定量PCR 验证，结果表明26 个基因的qRT-PCR 表达丰度分析与
cDNA-AFLP 结果一致。分离到植物生长素、细胞分裂素、油菜素内酯、脱落酸、细胞周期等调控途径中
生长调控相关的候选基因，可为阐明节间生长调控机制奠定基础。

关键词: 桃, 半矮生型, cDNA-AFLP, 差异表达基因, 节间长度, 功能注释

Abstract: This study aimed to select and identify genes related to regulate the length of internode，
and provide basis for revealing expression pattern of regulating internode length related genes and cloning
the gene of controlling semi-dwarf trait. cDNA-AFLP technique was employed to investigate differentially
expressed genes. The transcript-derived fragments（TDFs）was recycled，cloned，sequenced and functionally
annotated and then validate by qRT-PCR. A total of 9 021 TDFs were screened by 256 primer
combinations，of which 899 TDFs were identified differentially expression. Totally，497 TDFs were
successfully sequenced and 254 sequences were high similar to genes with known functions. The results
indicated that these genes were involved in signal transduction，growth and development，transcription
regulation，stress tolerance，protein degradation and synthesis and so on. The expression abundance of 26
genes validated by real-time quantitative RT-PCR was consistent with the result of cDNA-AFLP analysis.
We generated candidate genes in plant IAA，CK，BR，ABA and cell cycle regulation pathways，which will
help to elucidate the molecular regulation mechanism of internode length.

Key words: peach, semi-dwarf type, cDNA-AFLP, differentially expressed genes, internode length, function annotation

中图分类号:

S 662.1

鲁振华, 李钰婷, 王志强*, 牛良, 崔国朝, 王玉兰. 半矮生型桃节间长度相关基因的筛选和鉴定[J]. 园艺学报, 2013, 40(5): 943-.

LU Zhen-Hua, LI Yu-Ting, WANG Zhi-Qiang-*, NIU Liang, CUI Guo-Chao, WANG Yu-Lan. Screening and Identification of Genes Involved in Regulating Internode Length for Semi-dwarf Peach[J]. ACTA HORTICULTURAE SINICA, 2013, 40(5): 943-.

[1]	翟含含, 翟宇杰, 田义, 张叶, 杨丽, 温陟良, 陈海江. 桃SAUR家族基因分析及PpSAUR5功能鉴定[J]. 园艺学报, 2023, 50(1): 1-14.
[2]	袁馨, 徐云鹤, 张雨培, 单楠, 陈楚英, 万春鹏, 开文斌, 翟夏琬, 陈金印, 甘增宇. 猕猴桃后熟过程中ABA响应结合因子AcAREB1调控AcGH3.1的表达[J]. 园艺学报, 2023, 50(1): 53-64.
[3]	邢柱东, 吕福堂, 郭尚敬, 张演义. 新品种‘聊大红金’桃[J]. 园艺学报, 2023, 50(1): 225-226.
[4]	杨兴旺, 王海波, 王莹莹, 王小龙, 王志强, 刘培培, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农甘爽’[J]. 园艺学报, 2022, 49(S2): 15-16.
[5]	杨兴旺, 王海波, 王莹莹, 张艺灿, 王宝亮, 刘培培, 史祥宾, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农白干’[J]. 园艺学报, 2022, 49(S2): 17-18.
[6]	杨兴旺, 刘凤之, 王海波, 王莹莹, 王志强, 史祥宾, 冀晓昊, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农寒水蜜’[J]. 园艺学报, 2022, 49(S2): 19-20.
[7]	杨兴旺, 刘凤之, 王海波, 王莹莹, 张艺灿, 李鹏, 王小龙, 刘万春, 王孝娣. 晚熟抗寒桃新品种‘中农秋香’[J]. 园艺学报, 2022, 49(S2): 21-22.
[8]	王莹莹, 刘立常, 刘志伍, 杨兴旺, 刘万春, 王孝娣, . 极晚熟桃新品种‘中农冬蜜’[J]. 园艺学报, 2022, 49(S2): 23-24.
[9]	王莹莹, 刘立常, 刘志伍, 杨兴旺, 刘万春, 王孝娣, . 小果油桃新品种‘中农珍珠’[J]. 园艺学报, 2022, 49(S2): 25-26.
[10]	吴延军, 刘庆忠, 陈鸿才, 戚行江, 朱东姿, 郑家祥, 曹学敏, 方丹燕. 甜樱桃新品种‘江南锦’[J]. 园艺学报, 2022, 49(S2): 29-30.
[11]	张晓明, 闫国华, 周宇, 王晶, 段续伟, 吴传宝, 张开春. 甜樱桃砧木新品种‘京春2号’[J]. 园艺学报, 2022, 49(S2): 31-32.
[12]	宋放, 陈奇, 袁炎良, 陈沙, 尹海军, 蒋迎春, . 黄肉猕猴桃新品种‘先沃1号’[J]. 园艺学报, 2022, 49(S2): 47-48.
[13]	齐永杰, 高正辉, 马娜, 王清明, 柯凡君, 陈钱, 徐义流, . 黄肉抗溃疡病猕猴桃新品种‘皖农金果’[J]. 园艺学报, 2022, 49(S2): 49-50.
[14]	张慧琴, 楼国荣, 陆玲鸿, 古咸彬, 宋根华, 谢鸣. 黄肉中华猕猴桃新品种‘金义’[J]. 园艺学报, 2022, 49(S2): 51-52.
[15]	季琳琳, 陈素传, 吴志辉, 常君, 韩文妍, 陶汝鹏. 早花山核桃新品种‘宁国山核桃2号’[J]. 园艺学报, 2022, 49(S2): 53-54.