关键词: 甜瓜, BADH 基因, 克隆, 非生物胁迫, 表达

Abstract: Abstract：The muskmelon（Cucumis melo L.）of TS-2 was used as experimental materials，using PCR
degenerate primers designed based on the conserved amino acid sequences of known betaine aldehyde
dehydrogenase to amplify cDNA fragments from muskmelon by RT-PCR and 3′，5′RACE，a BADH gene
named CmBADH was cloned and the registration number in GenBank is JN091961.1. Bioinformatics
analysis indicated that the full length sequence of BADH was 1 856 bp，encoding 503 amino acids residues
with a calculated molecular weight of 54 kD and isoelectric point of 5.182. The BADH protein in
muskmelon showed the highest similarity with from Jatropha curcas，and the similarity was 82.96%.
There were four strong inside to outside transmembrane helixes. PlantCare analysis indicated that there
were cis-acting responsive elements of MeJA-responsiveness，defense and stress，zein metabolism
regulation，low-temperature，light responsiveness，meristem expression，and circadian control as well as
MYB binding site involved in drought-inducibility. The result of RT-PCR analysis indicated that the
expression of CmBADH is induced by salt stress，drought stress，low temperature and high temperature
stress，and its expression trend increased firstly and then decreased the expression of CmBADH was also
induced by ABA treatment.

Key words: Cucumis melo L., BADH gene, cloning, abiotic stress, expression