关键词: 黄瓜, 果实弯曲, Cs14-3-3 基因, 表达分析

Abstract: Abstract：By RT-PCR technology，we separated and cloned the 14-3-3 protein gene from the peel of
the cucumber（Cucumis sativus L.）bending variety‘Changchunmici’，named Cs14-3-3．The full length
cDNA of Cs14-3-3 is 792 bp，encoding a protein of 263 amino acids．Its molecular mass is 29 589.287 Da，
and pI is 4.585. Bioinformatic analysis showed that the protein of Cs14-3-3 possesses the basic structure of
14-3-3 proteins，and shares high homology with the known 14-3-3 proteins，which is belonging to the
14-3-3 protein family. At the same time，by using real-time fluorescence quantitative PCR（RT-qPCR），
we studied the expression of mRNA in straight fruit and the abdomen and the ridge of the bending fruit in
the periods of 2、4、6、8、10、12 days after flowering. The result showed that in each period，the expression
level of Cs14-3-3 from high to low order is bending fruit abdomen，straight fruit，bending fruit ridge，and
in each part，the expression level of Cs14-3-3 in 2 days after flowering obviously higher than that in the
other periods. In conclusion，it was preliminarily revealed that Cs14-3-3 gene is related to fruit bending in
cucumber，and plays an important role in the period of early fruit development after flowering.

Key words: cucumber, fruit bending, Cs14-3-3 gene, expression analysis