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园艺学报 ›› 2013, Vol. 40 ›› Issue (4): 724-.

• 研究报告 • 上一篇    下一篇

梨两个休眠相关MADS-box 基因特征及在其休眠过程中的表达分析

 刘国琴, 郑鹏华, Sayed Hussain, 滕元文   

  1. (1 浙江大学园艺系,农业部园艺植物生长发育与品质调控重点开放实验室,杭州 310058;2 贵州大学农学院,贵阳
    550025)
  • 出版日期:2013-04-25 发布日期:2013-04-25

Characteristics of Two Dormancy-associated MADS-box Genes in Pear and Their Expression Analysis During the Dormancy

 LIU  Guo-Qin, ZHENG  Peng-Hua, Sayed  Hussain, TENG  Yuan-Wen   

  1. (1Department of Horticulture,State Agricultural Ministry Key Laboratory of Horticultural Plant Growth,Development &
    Quality Improvement,Zhejiang University,Hangzhou 310058,China;2 College of Agriculture,Guizhou University,
    Guiyang 550025,China)
  • Online:2013-04-25 Published:2013-04-25

摘要: PpMADS1 PpMADS2 是从‘酥梨’(Pyrus pyrifolia white pear group‘Suli’)休眠芽转录组
文库筛选的两个与休眠相关的MADS-box 基因序列。为了解序列的特征,对其进行了相关生物信息学分
析,并以‘翠冠’和‘圆黄’梨为试材,用实时定量PCR 技术分析其花芽休眠不同阶段的表达变化。结
果表明:两个基因都具有MADS-box 家族的特征序列MIKC-基序,PpMADS1 PpMADS2 分别与日本梨
P. pyrifolia‘Kosui’)休眠相关的两个MADS-box 基因PpMADS13-1PpMADS13-2 聚在一起,且单独
聚为一支,与李属植物休眠相关的MADS-box 基因关系最近。在两个品种的休眠过程中,PpMADS1
PpMADS2 的表达呈现相似的变化趋势,都有一个表达高峰,但‘翠冠’梨PpMADS1 PpMADS2 的表
达高峰均出现在11 月15 日,而‘圆黄’梨PpMADS1 的表达高峰延后到12 月30 日,PpMADS2 的表达
高峰出现在12 月15 日。两个品种PpMADS1PpMADS2 的表达高峰都出现在内休眠解除之前,随内休
眠解除其表达量下调,在生态休眠阶段表达量维持在较低的水平。据此推测PpMADS1 PpMADS2 的表
达对梨芽内休眠的解除具有调控作用

关键词: 梨, 芽, 休眠, MADS-box 基因, 表达分析

Abstract: Two dormancy-associated MADS-box genes(PpMADS1 and PpMADS2)were obtained
from transcriptome library constructed Pyrus pyrifolia white pear group‘Suli’. To understand sequence
characteristics of two genes,bioinformatics analysis was carried out. Furthermore the gene expression
patterns in lateral flower buds of both pear cultivars‘Cuiguan’and‘Wonhwang’were analyzed during the
period of dormancy by quantitative real-time PCR. The results showed that PpMADS1 and PpMADS2
contained well-conserved MIKC-motifs of MADS-box. A phylogenetic analysis revealed that PpMADS1
was closely related to PpMADS13-1 of Japanese pear(P. pyrifolia‘Kosui’),whereas PpMADS2 was
similar to PpMADS13-2 of Japanese pear. The pear DAM genes formed an independent subclade and were
closely related to those of Prunus spp. The expression patterns of PpMADS1 and PpMADS2 were similar
with one peak in two cultivars during the period of dormancy. However,the timing of gene expression
peak differed between two cultivars. The PpMADS1 and PpMADS2 expression peak occured on the
November 15th in‘Cuiguan’pear,while the PpMADS1 expression peak on the December 30th,and the
PpMADS2 expression peak on the December 15th in‘Wonhwang’pear. The PpMADS1 and PpMADS2
expression peak appeared prior to the release of endodormancy,and their expression levels were gradually
down-regulated after the turn over of endodormancy and kept at a lower level during the period of
ecodormancy. These results indicated that PpMADS1 and PpMADS2 might play an important role in the
regulation of bud endodormancy-release in pear.

Key words: pear, bud, dormancy, MADS-box gene, expression analysis

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