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园艺学报 ›› 2013, Vol. 40 ›› Issue (4): 641-.

• 果树 • 上一篇    下一篇

‘红颜’草莓菌根磷转运蛋白基因的克隆及荧光定量表达分析

 曹庆芹, 邓 杰, 朱丽静, 白隽帆, 赵 天, 朱旭文, 姜奕晨   

  1. (1 北京农学院生物科学与工程学院,北京 102206;2 农业应用新技术北京市重点实验室,北京农学院植物科学技术
    学院,北京 102206)
  • 出版日期:2013-04-25 发布日期:2013-04-25

The Cloning and Quantitative Expression Analysis of Mycorrhizal
Phosphate Transporter Gene in Fragaria × ananassa

CAO  Qing-Qin, DENG   Jie, ZHU  Li-Jing, BAI  Juan-Fan, ZHAO   Tian, ZHU  Xu-Wen, JIANG  Yi-Chen   

  1. (1 College of Biological Science and Engineering,Beijing University of Agriculture,Beijing 102206,China;2 Beijing Key
    Laboratory for Agricultural Application and New Technique,College of Plant Science and Technology,Beijing University of
    Agriculture,Beijing 102206,China)
  • Online:2013-04-25 Published:2013-04-25

摘要: 采用MEGA5.0 软件对9 个森林草莓无机磷转运蛋白序列及58 个已知的其它植物磷转运蛋
白的氨基酸序列进行聚类分析,发现5 个基因与菌根磷转运蛋白基因分支聚在一起,初步判断是候选的
草莓菌根磷转运蛋白。根据其基因序列设计引物获得了5 个‘红颜’草莓候选菌根磷转运蛋白基因全长。
实时荧光定量PCR 结果表明,FaPT4FaPT5 FaPT8 在草莓菌根中显著上调表达,结合聚类分析结果,
证明这3 个基因是‘红颜’草莓菌根磷转运蛋白基因。

关键词: 草莓, 菌根磷转运蛋白基因, 基因克隆, 聚类树, 荧光定量表达分析

Abstract: Phylogenetic tree was clustered by MEGA5.0 based on amino acid sequences of nine
Fragaria vesca inorganic phosphate transporters and 58 known phosphate transporters of other plants. The
results showed five inorganic phosphate transporters in Fragaria vesca were gathered the branch of
mycorrhizal phosphate transporter,so these five genes were preliminarily predicted candidates of
mycorrhizal phosphate transporter. Based on homologous sequences,full sequences of these five genes in
Fragaria × ananassa Duch.‘Benihoppe’were obtained. Real-time quantitative PCR showed that,FaPT4,
FaPT5 and FaPT8 genes were dramaticly induced in the mycorrhizal roots of strawberry combined with
cluster result above. Therefore,FaPT4,FaPT5 and FaPT8 were proven to be mycorrhizal phosphate
transporter genes in Fragaria × ananassa Duch.‘Benihoppe’.

Key words: Fragaria ×, ananassa, mycorrhizal phosphate transporter gene, gene cloning, phylogenetic tree, quantitative expression

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