江苏省番茄黄化曲叶病的病原分子诊断

园艺学报 ›› 2008, Vol. 35 ›› Issue (12): 1815-1818.

江苏省番茄黄化曲叶病的病原分子诊断

季英华¹;熊如意¹;程兆榜¹;周彤¹;赵统敏²;余文贵²;范永坚¹;周益军^1*

(¹江苏省农业科学院植物保护研究所，南京 210014；²江苏省农业科学院蔬菜研究所，南京 210014)

收稿日期:2008-05-22 修回日期:2008-11-03 出版日期:2008-12-25 发布日期:2008-12-25
通讯作者: 周益军

Molecular Diagnosis of Tomato Yellow Leaf Curl Disease in Jiangsu Province

JI Ying-hua¹,XIONG Ru-yi¹,CHENG Zhao-bang¹,ZHOU Tong¹,ZHAO Tong-min²,YU Wen-gui²,FAN Yong-jian¹,and ZHOU Yi-jun^1*

(¹Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; ²Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)

Received:2008-05-22 Revised:2008-11-03 Online:2008-12-25 Published:2008-12-25
Contact: ZHOU Yi-jun

摘要/Abstract

摘要：

2007年冬季在江苏省兴化市和南京市一些日光温室大棚中发生了一种新的番茄病毒病，主要表现为植株矮缩，叶片上卷，叶缘黄化等，危害十分严重。为明确其病原，对田间表现典型症状的20份病样进行了分子检测，结果全部检测到粉虱传双生病毒；对其中12份进行序列同源性分析，发现其同源性超过98%，说明其极可能是一个分离物；将该序列与Genbank进行BLAST分析，发现其与番茄黄化曲叶病毒的同源性最高，达99％。

关键词: 番茄, 番茄黄化曲叶病, 粉虱传双生病毒, 分子诊断

Abstract:

In the winter of 2007, a new virus disease on tomato broke out in Jiangsu and caused great losses to local tomato production. The infected tomato showed severely stunted, upward curling and prominent yellowing along margins and interveinal regions. Based on its symptoms and the occurrence of Bemisia tabaci in the fields, Whitefly-transmitted geminivirus (WTG) was suspected to be the pathogen, and WTG was identified in all the 20 samples. The nucleotide sequence identities between these isolates were extremely high (over 98.9%), which means no complex infection was found. The result of BLAST revealed they shared high sequence identities (over 99%) with a member of Begomovirus: Tomato yellow leaf curl virus (TYLCV).

Key words: tomato, tomato yellow leaf curl disease, whitefly-transmitted geminivirus, molecular diagnosis

中图分类号:

S 641.2

季英华;熊如意;程兆榜;周彤;赵统敏;余文贵;范永坚;周益军. 江苏省番茄黄化曲叶病的病原分子诊断 [J]. 园艺学报, 2008, 35(12): 1815-1818.

JI Ying-hua;XIONG Ru-yi;CHENG Zhao-bang;ZHOU Tong;ZHAO Tong-min;YU Wen-gui;FAN Yong-jian;and ZHOU Yi-jun. Molecular Diagnosis of Tomato Yellow Leaf Curl Disease in Jiangsu Province[J]. ACTA HORTICULTURAE SINICA, 2008, 35(12): 1815-1818.

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