关键词:

茄子, 花青素, SmMYB 基因, 表达分析

Abstract: MYB gene’s cDNA and gDNA sequences in full length were cloned from eggplant
（Solanum melongena L.） cultivars‘YZ14’and‘YZ3’using RT-PCR and RACE. Sequence analysis shows
that the full length of cDNA is 1 035 bp long，and the open reading frame（ORF）is 837 bp long，encoding
278 amino acids. The protein isoelectric point is at pH8.47. The SmMYB protein contains two typical
DNA-binding domains and has 71% homologies with anthocyanin biosynthesis-related R2R3-MYB
transcription factor in capsicum. Furthermore，the protein is located mostly in the nucleus，in agreement
with the regulatory function of MYB transcription factors. The corresponding gDNA is 3 834 bp in length，
consisting of three exons and two introns. Fluorescence semi-quantitative PCR analysis indicates that
SmMYB is expressed in all organs of the plant，including roots，stems，leaves，petals，and peels. However，
the expression level of SmMYB is tissue-specific，and highly correlated with the concentration of
anthocyanin in peel after shading treatment. Therefore，the SmMYB is speculated to be a MYB transcription factor gene，which positively regulates the biosynthesis of anthocyanin in eggplant.

Key words: eggplant, anthocyanin, SmMYB gene, expression analysis