关键词: 芜菁, COP1, 克隆, 表达特性

Abstract: The COP1 gene（BrCOP1，accession number JF738111）was isolated from total RNA of Brassica rapa L. ssp. rapifera‘Tsuda’by RT-PCR and RACE. BrCOP1 cDNA contained an opening reading frame of 2 034 bp，encoding a predicted protein of 677 amino acid residues. The deduced amino acid sequence of BrCOP1 shared 94% identity with Arabidopsis thaliana COP1. Northern blotting results demonstrated that expression of BrCOP1 exhibited no tissue specificity，and had no obvious difference in seedlings under various treatments but was induced by UV-A light，while the expression of BrCOP1 was induced after exposed to UV-A for 6 hours in the swollen hypocotyls，however expression level was not increased with the prolongation of time. The above results suggested that BrCOP1，as a key factor of light-dependent development，might play a crucial role in UV-A induced anthocyanin biosynthesis and light signal transduction.

Key words: turnip, COP1, cloning, expression analysis