关键词: 甘蓝, 授粉, 花粉—柱头相互作用, 蛋白质组学, 双向电泳

Abstract: In order to study the early pollen-stigma interaction in Brassica oleracea through proteomics analysis，inbred line A₄ was pollinated with inbred line K₁₀₁. Total stigma/pollen proteins were extracted by TCA/acetone at 3–5 min，1 h and 2 h after pollination，respectively. Total proteins were separated by two-dimensional gel electrophoresis and analyzed through MALDI-TOF-TOF-MS mass spectrometry. 116 proteins were differentially expressed contrasted the total stigma/pollen proteins of 1 h and 3–5 min after pollination，but only 7 proteins expressed differentially contrasted the total stigma/pollen proteins of 1 h and 2 h after pollination. 71 protein spots were analyzed through MALDI-TOF-TOF-MS mass spectrometry，and 31 proteins were identified homologous to known proteins by MASCOT analysis. Compared with total stigma/ pollen proteins of 3–5 min after pollination，19 of 31 identified proteins were specifically expressed 1 h after pollination，9 up-regulated and 3 down-regulated ones. The predicted functions of the 31 protein were related to pollen tube development，Ca²⁺ binding，biosynthetic process，ransmembrane transport，defense response，protein folding，carbohydrate and energy metabolism，regulation of translation and methylation. This study has documented the dynamics of protein expression during the early pollen-stigma interaction in Brassica oleracea and provides insights into the fundamental mechanisms involved in these processes.

Key words: Brassica oleracea L., pollination, pollen-stigma interaction, proteomics, 2-DE