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园艺学报 ›› 2008, Vol. 35 ›› Issue (12): 1717-1726.

• 果树 •    下一篇

中国板栗3个野生居群部分表型性状的遗传多样性

马玉敏1,2; 陈学森*1;何天明3;吴传金1;王娜1   

  1. 1山东农业大学作物生物学国家重点实验室,泰安 271018;2泰安市泰山林业科学研究院,泰安 271000;3新疆农业大学,乌鲁木齐 830052)
  • 收稿日期:2008-07-16 修回日期:2008-10-14 出版日期:2008-12-25 发布日期:2008-12-25
  • 通讯作者: 陈学森

Genetic Diversity of Morphological Traits in Wild Populations of Castanea mollissima Blume

MA Yu-min1,2,CHEN Xue-sen1,HE Tian-ming3,WU Chuan-jin1,WANG Na1   

  1. (1State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018,China; 2 Mountains Tai Institute of Forestry ,Tai'an 271000;3 Xinjiang Agricultural University , Wulumuqi 830052,China)
  • Received:2008-07-16 Revised:2008-10-14 Online:2008-12-25 Published:2008-12-25
  • Contact: CHEN Xue-sen

摘要: 本文对中国野生板栗秦岭山脉生态区、泰沂山脉生态区和燕山山脉生态区的3个种下居群的叶片、果实形态和坚果主要营养成分进行了调查研究,旨在为我国野生板栗资源的保护和可持续利用提供科学依据。结果表明,①3个居群的叶片大小、叶柄长度和粗度以及果实形状、大小和颜色等形态性状的变异系数均在10%以上,表现出丰富的遗传多样性,其中以叶面积及单粒重变异幅度和变异系数最大,而果形指数变异幅度和变异系数最小,是较稳定的植物学性状,3个居群的变异趋势基本一致。本文首次对秦岭山脉野生板栗表型性状进行了调查研究,其中单果重的变异幅度为1.69-3.89g,变异系数18.3%;坚果中边果的形状有近圆形、圆形、椭圆形、扁椭圆形、短椭圆形等;坚果表面颜色有淡红、棕褐、红褐、褐色等;果肉有浅黄色、乳黄色等;涩皮均易剥离,具有北方炒食板栗的典型特征。② 3个居群118个野生株系果肉中水分、总糖、淀粉、蛋白质、脂肪、灰分和维生素C等各种主要营养成分的含量在单株间差异显著,变异系数6.2%-28.3%,其中以坚果蛋白质含量的变异幅度和变异系数最大,遗传多样性丰富,3个居群的变异趋势基本一致;③同一性状在不同居群间大都存在显著或极显著差异,其中单叶面积和单粒重以秦岭山脉居群最小,分别为56.34cm2和2.95g,而维生素C含量最高(96.7mg/100g),分别是燕山山脉和泰沂山脉居群的2.29倍和2.22倍。④相关分析结果显示,单粒重和单叶面积与降雨量和经度显著正相关,与海拔高度显著负相关,而维生素C含量与降雨量显著负相关,与海拔高度正相关,表明环境对板栗表型性状的遗传变异具有较大影响。因此,从野生板栗资源中进一步选择大果型、高蛋白质及高维生素C含量等优异种质类型的潜力很大。

关键词: 中国板栗, 表型, 遗传多样性, 变异系数

Abstract: The diversity of leaf, petiole and nut in 3 wild intraspecific populations (the Qinling Mountains ecotype, the Taiyi Mountains ecotype, and the Yanshan Mountains ecotype) of Castanea mollissima Blume were investigated to provide the scientific basis for resource protection and utilization. The results indicated: ①There were significant difference among and within population. The morphological traits, such as leaf length, width, leaf shape index, length of petiole and nut shape, size, color, have great morphological diversity with variation coefficient (CV) of more than 10%. The range of nut weight varied from 1.69g to 3.89g in Qinling population with CV 18.3%. The range and CV of leaf area and nut weight were the largest, and those of leaf shape index were the smallest; ②The content, of main nutrition composition (water, total sugar, starch, protein, fat, ash and Vc) in ripe nuts determined in obtained 118 seedlings at the Qinling Mountains, Taiyi Mountains and Yanshan Mountains was analyzed and presented considerable genetic variations with CV of 6.2%-28.3%, and the range and CV of the protein were the largest. There were the same trends in the 3 wild intraspecific populations; ③There was significant difference between site conditions, with smallest CV in leaf area and nut weight and highest CV of Vc content; ④Correlation analysis revealed that mean weight of a single nut with annual rainfall and the longitude of each ecotype, in contrast, they were negatively correlated with the altitude. Vitamin C contents in the nuts from different ecotype were negatively regulated with annul rainfall. These relationships indicated that environmental conditions had impact on genetic diversity in Chinese wild chestnut.

Key words: Castanea mollissima Blume, Morphological character, Genetic diversity, Variation

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