关键词: 月季, 乙烯, 乙烯受体, 基因克隆, 原核表达

Abstract: In this paper，twenty-two UniGene fragments that shared high homology with ethylene receptor genes were obtained from previous-constructed rose flower transcriptome database. Then the full length of three genes，RhETR1，RhETR3，and RhETR5，were cloned from rose petals. The full length and encoded peptide chain of RhETR1，RhETR3，and RhETR5 were 2 814 bp and 595 aa，2 468 bp and 765 aa，and 2 740 bp and 742 aa，respectively. The predicted protein of the three genes shared the highest identity with ZjETR1 in Ziziphus jujuba（85.2%），PtETR2 in Populus trichocarpa（75.8%），andPpERS2 in Pyrus pyrifolia（80.3%），respectively. Further protein homologous and construction analysis indicated that RhETR1 andRhETR5were similar to ERS1andETR1in Arabidopsis，belonging to subfamily Ⅰ；RhETR3 was similar to ERS2，belonging to subfamily Ⅱ. During natural vasing life，RhETR1，RhETR3，and RhETR5 showed down-regulated，up-regulated and constitutive expression，respectively. Through a prokaryotic expression system，the recombinant protein of RhETR3 was obtained，whose molecular weight was similar to that of the theoretical ones，indicating that the cloned full-length of RhETR3 has a complete open reading frame.

Key words: rose, Rosa hybrida, ethylene, ethylene receptor, gene clone, prokaryotic expression