关键词: 番茄, RILs 群体, AFLP 标记, 荧光标记引物, 遗传连锁图谱

Abstract: A genetic linkage map of tomatowas constructed using a RILs（recombinant inbred lines）population of 80 individuals which was developed by crossing Solanum lycopersicum 99165-30 andSolanum habrochaites LA1777 through single-seed descent（SSD）. AFLPs were generated by the use of restriction enzymes EcoRⅠin combination with either MseⅠ. Pre-amplification was carried out using primers corresponding to EcoRⅠ and MseⅠ adaptors with no selective base. Selective amplifications were performed using IRD700 or IRD800 labeled EcoRⅠ primers and non-labeled MseⅠ primers. The resulting products were denatured in formamide at 95 ℃ and separated by electrophoresis 2.5 h on 6% polyacrylamide gel using IR² DNA Analyzer（Model 5200 LI-COR Biosciences，Lincoln，NE）. The segregation of each marker and linkage analysis was done using the program JoinMap version 3.0. With 22 primer pairs，a total of 247 parental polymorphic bands were detected and 125 used for mapping，the total map length was 662 cM，consisted of 18 linkage groups，number of markers in the linkage groups varied from 3 to 22，the length of linkage groups were from 14.0 cM to 58.0 cM and mean marker interval distance from 2.27 cM to 13.3 cM individually，and a mean marker interval distance of 5.3 cM between markers. The map developed in the present study could be used for genetic mapping and molecular marker assisted breeding and quantitative trait locus mapping of tomato.

Key words: tomato, recombinant inbred line, AFLP, IRD700 or IRD800 labeled EcoRⅠprimers, genetic linkage map