部分矮牵牛品种亲缘关系的SRAP分析

园艺学报 ›› 2008, Vol. 35 ›› Issue (12): 1837-1842.

部分矮牵牛品种亲缘关系的SRAP分析

徐进¹;张西西^1,2;董爱香²;王涛²;赵梁军^1*

（¹中国农业大学观赏园艺与园林系，北京100094；²北京市园林科学研究所，100102）

收稿日期:2008-06-06 修回日期:2008-10-21 出版日期:2008-12-25 发布日期:2008-12-25
通讯作者: 赵梁军

Genetic Relationship of Some Cultivars of Petunia hybrids Using SRAP Marker

XU Jin¹, ZHANG Xi-xi^1,2, DONG Ai-xiang², WANG Tao²,and ZHAO Liang-jun^1*

(¹Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100094，China;²Beijing Institute of Horticulture and Gardening, Beijing 100102, China)

Received:2008-06-06 Revised:2008-10-21 Online:2008-12-25 Published:2008-12-25
Contact: ZHAO Liang-jun

摘要/Abstract

摘要：

本文首次利用分子标记SRAP(sequence-related amplified polymorphism)对部分矮牵牛品种进行亲缘关系分析。采用88对引物对我国栽培的58个矮牵牛品种进行扩增，从中筛选得到20个多态性较高的引物组合，共产生389条多态性条带，平均每个引物组合产生19.5个多态性条带，每个引物组合分别产生13～40个扩增带，Jaccard's相似系数在0.55～0.87之间。在遗传距离(GD)为0.67处，可将58个矮牵牛品种分为4个类群，第一类群主要为暖色系列的品种，第二类群为黄色花品种，第三、四类群为冷色花品种，白色花品种聚类较散。本研究结果为开展矮牵牛新品种保护、种子纯度检测以及分子育种提供了分子生物学资料。

关键词: 矮牵牛, SRAP, 亲缘关系

Abstract: In this paper, the molecular marker system-SRAP (sequence-related amplified polymorphism) was firstly applied on 58 Petunia hybrids' genetic relationship research, the results obtained was as follows: 20 primer pairs selected from 88 primer pairs amplified 389 polymorphic bands with an average of 19.5 polymorphic bands per primer pair, and the number of bands amplified by each primer pair was ranged from 13 to 40. Moreover, Jaccard's similarity coefficient ranged from 0.55 to 0.87. 58 varieties could be divided into 4 groups when the genetic distance was 0.67. The first group mainly included warm colour serieses, and yellow flower variety was in the second group, while cold colour varieties were clustered in the third and forth group. In addition, the white colour varieties were clustered in a wide range. Study on genetic relationship of Petunia is benefit for variety protection, seed purity testing and genetic breeding.

Key words: petunia hybrida, SRAP, genetic relationship

中图分类号:

S 681

徐进;张西西;董爱香;王涛;赵梁军. 部分矮牵牛品种亲缘关系的SRAP分析[J]. 园艺学报, 2008, 35(12): 1837-1842.

XU Jin;ZHANG Xi-xi;DONG Ai-xiang;WANG Tao;and ZHAO Liang-jun. Genetic Relationship of Some Cultivars of Petunia hybrids Using SRAP Marker [J]. ACTA HORTICULTURAE SINICA, 2008, 35(12): 1837-1842.

[1]	张萌, 单玉莹, 杨业波, 翟飞飞, 王兆山, 巨关升, 孙振元, 李振坚. 中国石斛属植物遗传资源的AFLP分析[J]. 园艺学报, 2022, 49(6): 1339-1350.
[2]	吴致君, 李伟, 王兴华, 张成, 蒋勋, 黎盛, 罗理勇, 孙康, 曾亮. 基于SLAF-seq的茶组子房室数不稳定居群种质亲缘关系分析[J]. 园艺学报, 2022, 49(11): 2455-2470.
[3]	王鑫, 李明阳, 田琳, 刘冬云. 利用ISSR标记及rDNA-ITS序列分析河北省野生铁线莲的亲缘关系[J]. 园艺学报, 2021, 48(9): 1755-1767.
[4]	田绍泽1，刘思禹1,2，王坤1，岳远征1,3，马方芳1，杨赵楠1，殷倩1，胡惠蓉1,*. 矮牵牛花发育相关基因PhTs2的克隆及功能分析[J]. 园艺学报, 2020, 47(6): 1087-1097.
[5]	张慧琳，朱婉，田丽，张蔚*. 矮牵牛冷响应转录因子PhZPT2-12的特性及表达分析[J]. 园艺学报, 2019, 46(8): 1543-1552.
[6]	周琴1，鲁瑞1，张书婷1，包满珠1，刘国锋1,2,*. 一个矮牵牛花器官发育突变体aps的表型鉴定及遗传分析[J]. 园艺学报, 2019, 46(2): 317-329.
[7]	方茜，张园园，杨钰婷，黄苗苗，符巧丽，周慧莎，陈文荣，宗宇，郭卫东. 越橘叶片转录组SSR发掘及其多态性研究[J]. 园艺学报, 2018, 45(7): 1359-1370.
[8]	陈倩倩1,2，荣丽媛2，邵紫君2，刘甜2，魏蕾3,，宋振巧1,2,. 利用SRAP和EST-SSR分析香椿资源的遗传多样性[J]. 园艺学报, 2018, 45(5): 967-976.
[9]	吴志娟，方茜，李永强，陈文荣，宗宇，郭卫东. 越橘反转录转座子插入多态性分子标记开发及品种鉴别[J]. 园艺学报, 2018, 45(4): 753-763.
[10]	王丽媛，孙鹏，张嘉嘉，傅建敏*，刁松锋，韩卫娟，索玉静，张悦. 柿野生雄性资源调查及其遗传多样性研究[J]. 园艺学报, 2018, 45(2): 261-278.
[11]	梁玲鸽，杨霞，王浩，李政，李名扬，郭余龙*. 超量表达PhWRIL1基因抑制矮牵牛叶片外植体再生不定芽[J]. 园艺学报, 2018, 45(10): 1961-1969.
[12]	吴洋洋1，仰小东1，杨信程1，徐婷婷1，管志勇1，薛建平2，蒋甲福1，陈素梅1，房伟民1，陈发棣1，张飞1,*. 菊花不完全双列杂交F1代遗传关系的SRAP分析[J]. 园艺学报, 2017, 44(6): 1116-1124.
[13]	杨莎，张彬，韩垚，杨霞，李名扬，郭余龙. 矮牵牛PDS基因的克隆及其在shRNA**介导的基因沉默中的应用[J]. 园艺学报, 2017, 44(2): 315-322.
[14]	岳远征1,2，刘思禹2，殷超群2，郭芮2，杨梅2，田绍泽2，胡惠蓉2,*. 弱光和短日照下‘幻想’矮牵牛花芽代谢组学研究[J]. 园艺学报, 2017, 44(12): 2391-2398.
[15]	刘志洋*，陈曦. 矮牵牛新品种‘玫瑰红’[J]. 园艺学报, 2016, 43(S2): 2795-2796.