关键词: 百合, 铁炮百合, 热激转录因子, 克隆, 表达, 荧光定量RT-PCR

Abstract: A full length cDNA named LlHSF1，belonging to Heat shock transcription factor（HSF），was cloned from Lilium longiforum‘White Heaven’by RT-PCR and RACE（rapid amplification of cDNA ends）. The gene consisted of 1 068 bp and open reading frame encompassed 780 bp encoding a polypeptide of 260 amino acids with calculated protein molecular mass of 30.123 kD. Cluster analysis showed that this gene was highly homologous to known HSF from other organisms. RT-PCR analysis showed that expression of LlHSF1 could be detected in leaf，root and bulb at 22 ℃，and it was expressed highly in the leaf.Theresults with SYBR GREEN real-time quantitative PCR showed that the gene expression level increased in leaves during 1–12 hours at 42 ℃.

Key words: Lilium longiforum, heat shock transcription factor（HSF）, cloning, real-time quantitative PCR