芦笋超雄株的DNA 分子标记辅助筛选

园艺学报 ›› 2012, Vol. 39 ›› Issue (11): 2182-2188.

芦笋超雄株的DNA 分子标记辅助筛选

周劲松, 汤泳萍, 罗绍春, 谢启鑫, 张岳平, 陈光宇

（江西省农业科学院蔬菜花卉研究所，江西省农业科学院生物工程中心，南昌 330200）

出版日期:2012-11-25 发布日期:2012-11-25

Selection of Super-male Plants in Asparagus with DNA Molecular Marker

ZHOU Jin-Song, TANG Yong-Ping, LUO Shao-Chun, XIE Qi-Xin, ZHANG Yue-Ping, CHEN Guang-Yu

（Institute of Vegetables and Flowers/Biotechnology Center，Jiangxi Academy of Agricultural Sciences，Nanchang 330200，China）

Online:2012-11-25 Published:2012-11-25

摘要/Abstract

摘要： 以芦笋两性株自交S₁代雄株为试验材料，首先与优良雌株进行测交，然后利用与芦笋性别决定基因紧密连锁的显性DNA分子标记对苗期各单株进行性别鉴定，推断出超雄株，为芦笋全雄育种提供亲本材料。研究结果表明：显性标记Asp1-T7和Asp1-T7sp特异性好，扩增效果稳定，均能够鉴别芦笋雌、雄性别；一共测验了14株芦笋两性株自交S₁代的雄株，从中筛选出2株超雄株；对田间测交组合单株显花后性别表型调查的结果表明，DNA分子标记检测结果与田间实际相符，2株超雄株所对应的组合被确证为全雄组合。

关键词: 芦笋, 超雄株, DNA 分子标记辅助选择, 测交

Abstract: DNA molecular marker assisted selection of super-male plants was conducted for all-male breeding of asparagus.Males in S₁ population of andromonoecious plants were test-crossed with elite females. Two DNA molecular markers tightly linked to sex determining gene M，Asp1-T7 and Asp1-T7sp，which showed stable and specific amplification，were used for sex identification of individuals in the progeny at seedling stage.Putative‘supermales’（MM）were differentiated from the normal males（Mm） by the presence or absence of‘all-male’population in the progeny. Two out of 14 male plants test crossed were distinguished as putative‘supermales’. The test result of DNA molecular marker-assisted selection was highly consistent with that of the sexual phenotype in field survey.The corresponding combinations originated from the two super-male plants were also identified as all-male combinations.

Key words: Asparagus officinalis, super-male plant, DNA molecular Marker-Assisted Selection, testcross

中图分类号:

S 644.6

周劲松, 汤泳萍, 罗绍春, 谢启鑫, 张岳平, 陈光宇. 芦笋超雄株的DNA 分子标记辅助筛选[J]. 园艺学报, 2012, 39(11): 2182-2188.

ZHOU Jin-Song, TANG Yong-Ping, LUO Shao-Chun, XIE Qi-Xin, ZHANG Yue-Ping, CHEN Guang-Yu. Selection of Super-male Plants in Asparagus with DNA Molecular Marker[J]. ACTA HORTICULTURAE SINICA, 2012, 39(11): 2182-2188.

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